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从预处理的软木固体中克服光学纯乳酸生产的延长滞后阶段
Joana Campos1, Henrik Almqvist2, Jie Bao3
1Department of Chemical Engineering, Lund University, Lund, Sweden.
Frontiers in bioengineering and biotechnology
|September 25, 2023
概括
使用林氏纤维素生物质生产光学纯乳酸 (LA) 是高点聚乳酸 (PLA) 的关键. 一种新的方法克服了由预处理固体引起的发酵延迟,从而实现了高效的D-LA合成.
科学领域:
- 生物技术是生物技术.
- 生物精炼是生物精炼的一种方式.
- 聚合物科学 聚合物科学
背景情况:
- 光学纯净的乳酸 (LA) 对于产生具有增强热性质的晶体聚乳酸 (PLA) 至关重要.
- 在生物炼油厂的框架内,红纤维素生物质为LA生产提供了一个可持续的非食品替代品.
研究的目的:
- 通过使用基因工程 *P. acidilactici* ZP26.6 来研究从蒸汽预处理的软木固体中生产光学纯的D-乳酸 (D-LA).
- 为了识别和克服过程的局限性,特别是延长的滞后阶段,在同时糖化和发酵 (SSF) 过程中遇到.
主要方法:
- 培养P. acidilacticiZP26在与蒸汽预处理软木固体的同时糖化和发酵 (SSF) 过程中.
- 调查预处理固体对发酵动力学的影响,包括使用和不使用酶以及在模型葡萄糖介质中的实验.
- 在添加预处理固体和酶之前,通过在合成介质中启动发酵来减轻滞后阶段的策略.
主要成果:
- 添加预处理的软木固体 (7% w/w WIS) 诱导了发酵过程中的显著和长时间的滞后阶段 (>40小时).
- 这种滞后阶段独立于酶添加和模型发酵观察到,表明预处理固体直接抑制.
- 开发了一种成功的方法,在引入预处理的固体和酶之前,在合成介质中预培养微生物,以克服滞后阶段.
结论:
- 预处理的纤维素固体对微生物发酵构成重大挑战,导致延长的滞后阶段,阻碍有效的D-LA生产.
- 一种连续发酵策略,包括在合成介质中初步生长,然后添加预处理的固体和酶,有效地克服了这种抑制.
- 这种优化的SSF方法对用于先进的PLA应用的光纤生物质的光学纯D-LA的可持续和高效生产具有前景.
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