识别潜在的枢纽基因与免疫和代谢变化相关的在术后系统性炎症失调的潜在枢纽基因
Silu Cao1, Jinxuan Tang1, Miaomiao Fei1
1Department of Anesthesiology and Perioperative medicine, Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, China.
这项研究确定了关键基因,将免疫和代谢变化与手术后系统性炎症失调 (PSID) 患者联系起来. 这些发现为治疗这种复杂疾病提供了新的目标.
科学领域:
- 基因组学和系统生物学
- 免疫学和新陈代谢研究研究
背景情况:
- 术后系统性炎症失调 (PSID) 涉及相互关联的免疫和代谢异常.
- 鉴定在PSID中调解这些免疫代谢相互连接的枢纽基因至关重要,但仍未得到解决.
研究的目的:
- 确定负责PSID患者免疫和代谢变化之间的相互作用的枢纽基因.
- 在PSID的背景下探索这些枢纽基因的功能作用.
主要方法:
- 在PSID患者和对照患者的外周血液核细胞中分析了差异表达基因 (DEG).
- 利用相关性分析来探索与免疫相关的DEGs (irDEGs) 和与代谢相关的DEGs (mrDEGs) 之间的相互联系.
- 采用CytoHubba (Cytoscape) 和加权基因共同表达网络分析来选枢纽基因.
主要成果:
- 在PSID患者中确定了512个上调和254个下调的DEG.
- 基因丰富分析证实了与免疫和代谢途径的关联.
- 确定了14个独特的枢纽基因,CD28,CD40LG,MAPK14和S100A12被突出显示为在PSID中调解免疫代谢相互作用的关键;研究结果在PSID小鼠中得到了验证.
结论:
- 这项研究成功地确定了将PSID中的免疫和代谢变化联系在一起的枢纽基因.
- 这些发现为PSID中免疫代谢相互作用的机制提供了新的见解.
- 已识别的枢纽基因代表了PSID临床干预的潜在新目标.
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