在活跃和潜伏的结核病感染中,miR-146和miR-155的差异表达
Ebrahim Alijani1, Farhad Riazi Rad2, Asal Katebi2
1Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Iranian journal of public health
|September 25, 2023
概括
这项研究研究了结核病 (TB) 患者和潜在结核病感染 (LTBI) 个体的微RNA (miRNA) 水平. 米R-146和米R-155显示出作为结核病感染和激活的生物标志物的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 结核病 (TB) 仍然是一个重大的全球健康威胁.
- 潜伏性结核病感染 (LTBI) 影响了世界三分之一的人口.
- 特定的微RNA (miRNA),包括miR-21,miR-31,miR-146a和miR-155,都与免疫反应有关.
研究的目的:
- 为了研究miR-21,miR-31,miR-146a和miR-155.5的表达水平.
- 为了比较活跃结核病患者,LTBI个体和健康对照 (HC) 的外周血液单核细胞 (PBMC) 中的miRNA表达.
主要方法:
- 从研究参与者中分离了周围血液单核细胞 (PBMC).
- 用纯化蛋白衍生物 (PPD) 刺激PBMCs.
- 使用TaqMan逆转录量化聚合酶链反应 (RT-qPCR) 试验量化miRNA表达.
主要成果:
- 在PPD刺激后的活跃结核病患者中,miR-21和miR-31表达增加.
- 在PPD刺激后,miR-146表达在LTBI和活跃结核病组中都比HC高.
- 在活跃的结核病患者中,miR-155表达显著高于PPD刺激后的HC和LTBI组.
结论:
- miR-146和miR-155在结核病感染中起着至关重要的作用.
- 这些miRNA可以作为结核病感染和激活的潜在生物标志物.
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