来自临床批准的输液液体中的等离子体处理水溶液的抗癌作用,并补充有机分子
Valeria Veronico1, Sabrina Morelli2, Antonella Piscioneri2
1Department of Chemistry, University of Bari Aldo Moro, via Orabona, 4, 70126 Bari, Italy.
ACS omega
|September 25, 2023
概括
冷大气等离子体 (CAPs) 产生用于癌症治疗的活性氧和物种 (RONS). 在血处理水溶液 (PTWS) 中将CAP与l-tyrosine结合,有效地降低了癌细胞活力,并触发了细胞亡.
科学领域:
- 生物医学工程 生物医学工程
- 癌症研究 癌症研究
- 血医学是一种血医学.
背景情况:
- 寒冷的大气等离子体 (CAPs) 产生反应性氧和物种 (RONS),在细胞通路中至关重要.
- 有机分子,如,可以通过产生RONS表现出抗癌性质.
- 将血衍生RONS与有机分子结合起来用于协同的癌细胞攻击尚未被探索.
研究的目的:
- 为了研究血产生的RONS和l-tyrosine对癌细胞的联合疗效.
- 为了评估与氨酸配合的血处理水溶液 (PTWS) 对癌细胞活力和亡的影响.
- 阐明PTWS诱导的癌细胞死亡的潜在机制.
主要方法:
- 添加到生理盐溶液 (SIII) 中并暴露在血中.
- 由此产生的PTWS的有效性在四个癌细胞系 (SHSY-5Y,MCF-7,HT-29,SW-480) 上进行了测试.
- 分析了细胞活力,氧气吸收,细胞内活性氧物种 (ROS) 水平和亡途径.
主要成果:
- 在所有测试的癌细胞系中,PTWS显著降低了细胞活力和氧气吸收.
- 细胞内ROS水平增加,表明氧化应激增强.
- 观察到亡途径的激活,包括线粒体介导和p-JNK/caspase-3信号传递.
结论:
- CAPs与l-tyrosine的组合为癌症治疗提供了一个有希望的策略.
- 通过ROS生成和特定的信号通路,PTWS有效诱导癌细胞毒性和亡.
- 这种方法为开发有效的临床癌症疗法提供了一种新方法.
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