蝙蝠刺驱动抗RNA病毒先天免疫反应中的IFN-β生产
Feiyu Fu1, Qi Shao1, Jianjian Zhang1
1Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Frontiers in microbiology
|September 25, 2023
概括
巴西自由尾蝙蝠的干扰素基因刺激器 (STING) 对于激活对RNA病毒的干扰素反应至关重要. 这项研究证实了BatSTING的存在.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 遗传学 遗传学 是一个
背景情况:
- 干扰素基因刺激器 (STING) 在RNA病毒感染期间激活哺乳动物细胞的干扰素 (IFN) 反应.
- 在许多RNA病毒的宿主蝙蝠中,STING的作用仍然在很大程度上未被描述.
- 了解蝙蝠的刺功能对于了解鸟的病毒免疫力至关重要.
研究的目的:
- 在巴西自由尾蝙蝠 (Tadarida brasiliensis) 中识别和描述STING基因.
- 调查蝙蝠 (BatSTING) 在调解干扰素β (IFN-β) 生产和对抗RNA病毒的抗病毒反应中的作用.
- 阐明蝙蝠在先天性抗病毒免疫中的功能意义.
主要方法:
- 在T.I.的克隆. 巴西人 刺基因.刺基因.
- 在T.T.中过度表达和击倒BatSTING. 1个肺 (TB1 Lu) 细胞.
- 用cGAS进行刺激和感染膀性口腔炎病毒 (VSV-GFP),以评估抗病毒活性和IFN-β诱导.
主要成果:
- 过度表达BatSTING显著抑制了TB1 Lu细胞中的RNA病毒复制.
- 蝙蝠STING激活与基础IFN-β和IFN刺激基因 (ISG) 表达的调节有关.
- 击败BatSTING损害了IFN-β对VSV-GFP的反应,证实了其至关重要的作用.
结论:
- 蝙蝠STING对于诱导IFN-β表达和在蝙蝠中对RNA病毒产生抗病毒反应至关重要.
- 刺在蝙蝠对抗RNA病毒感染的天生的免疫力中发挥着关键的,非冗余的作用.
- 这些发现凸显了蝙蝠抗病毒防御机制中的STING的重要性.
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