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微型单胞菌深度TRM 95458将糖醇转化为一种新的透性化合物

Di Lu^1,2, Hong-Ling Shen^1,2, Lei Wang^1,2

¹Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Production & Construction Corps and The Ministry of Science & Technology, Tarim University, Alar, China.

Frontiers in microbiology

|September 25, 2023

概括

耐盐细菌Micromonospora profundi TRM 95458产生一种新的化合物ABAGG,以帮助植物在盐土壤条件下生存. 这一发现有助于在具有挑战性的环境中制定农业生产率战略.

科学领域:

微生物学微生物学
植物科学植物科学
生物化学生物化学

背景情况:

土壤的盐度和度显著限制了植物生长和农业生产率.
耐盐菌提供了一个有前途的策略,以提高植物对盐应激的弹性.
从小草根球中分离出来的微型胞深层TRM 95458,表现出适度的盐分耐受性.

研究的目的:

为了分离和表征一种由M. profundiTRM 95458.8.产生的新透性化合物.
研究生物合成途径和影响新化合物的因素.
评估该化合物在细菌适应盐条件方面的潜在作用.

主要方法:

使用NMR和HRMS的新化合物的隔离和结构阐明.
发酵研究,以分析糖醇转化为该化合物.
优化发酵条件,包括基质度 (甘油,甘氨酸) 和NaCl水平.

主要成果:

一种名为ABAGG的新透化合物被成功分离出来,并确定了它的结构.
M. profundi TRM 95458 证明了将糖醇转化为 ABAGG.的能力.
ABAGG积累受到糖醇,甘氨酸和NaCl度的影响,最佳产量在10 g/L NaCl.
研究人员发现,使用甘油和甘氨酸,盐应激会诱导ABAGG积累.

结论:

鉴定出ABAGG是一种由M. profundiTRM 95458.8产生的新型透化合物.
ABAGG的生物合成与盐应激下的甘油和甘氨酸代谢有关.
在盐环境中,ABAGG可能在M. profundi TRM 95458的生存和适应方面发挥着至关重要的作用.

关键词:

亚巴格 (Abagg) 是一个大城市.微型单胞体深层的 TRM 954588 的微粒体.兼容的溶解物是相容的转化糖醇的转化.耐盐的树根细菌耐盐的树根细菌.

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