在转基因根源上植入的非转基因番茄种子的多学科分析
Takumi Ogawa1,2, Kanae Kato2, Harue Asuka2
1Graduate School of Agriculture, Osaka Metropolitan University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Food safety (Tokyo, Japan)
|September 25, 2023
概括
将转基因植物部分移植到非转基因作物上可能会转移蛋白质和代谢物. 这项研究在非转基因番茄果实中发现了烟草蛋白和尼古丁,这引发了对移植产品的食品安全问题.
科学领域:
- 农业科学 农业科学
- 植物生物技术 植物生物技术
- 食品安全 食品安全
背景情况:
- 接种是一种常见的农业实践,用于结合理想的植物特征.
- 在非转基因作物上使用转基因 (GM) 植物部分进行移植,可能会产生具有非转基因可食部分的仿真植物.
- 种子和根茎之间的分子互换可以改变作物特性,需要对转基因移植进行风险评估.
研究的目的:
- 评估将转基因植物与非转基因作物移植的潜在风险.
- 为了研究巨分子和代谢物从根茎转移到种子在移植植物.
- 来自移植植物的非转基因食用部分的安全性.
主要方法:
- 准备异种移植 (转基因烟草根茎/非转基因番茄后代),同种移植 (非转基因番茄/非转基因番茄) 和异种移植 (非转基因烟草根茎/非转基因番茄后代) 线.
- 综合的多种OMIC分析非转基因番茄果实收获从接种线.
- 蛋白质组分析以检测物种间蛋白质转移和代谢分析的代谢概况.
主要成果:
- 蛋白质组分析证实了烟草衍生的蛋白质在异种移植和异种移植线路中转移到非转基因番茄果实.
- 过敏性番茄蛋白质 (Sola l 4.0101,Sola l 4.0201) 的转录水平在移植线下降.
- 从异种移植和异种移植线路的番茄果实中观察到烟草衍生的尼古丁的积累,这表明存在潜在的食品安全问题.
结论:
- 移植可以导致蛋白质和不利的代谢物 (例如,尼古丁) 从转基因根茎转移到非转基因可食部分.
- 外来蛋白质和代谢物的存在引起了对移植产品的食品安全考虑.
- 需要进一步的研究,以了解环境条件和生长周期对转移植物的非转基因食用部分的质量和安全性的影响.
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