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在结肠腺癌中基于miRNA-mRNA相互作用模式的预后特征的识别和验证

Qiwu Zhao¹, Haosheng Li¹, Wenchang Li²

¹Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Frontiers in cell and developmental biology

|September 25, 2023

概括

研究人员在结肠腺癌 (COAD) 中确定了关键的非编码RNA (ncRNA) 和它们的基因. 这一发现为COAD提供了潜在的新生物标志物和治疗点,改善了患者的预后和治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

非编码RNAs (ncRNAs),特别是microRNAs (miRNAs),通过形成RNA诱导沉默复合体 (RISC) 来调节结肠腺癌 (COAD) 中的基因表达.
在COAD中确定特定的miRNA生物标记物和治疗点仍然是临床应用的挑战.

研究的目的:

使用癌症基因组图谱 (TCGA) 数据集在COAD中选差异表达基因.
通过整合差异mRNA和miRNA表达数据来建立预后模型.
在COAD.中研究miRNA向基因的治疗潜力.

主要方法:

使用TCGA数据进行差异基因表达查.
使用基于最小绝对收缩和选择运算子 (LASSO) 的考克斯回归的预测模型开发.
在100名结直肠癌患者组织微阵列中对miRNA基因的免疫组合化学分析.

主要成果:

确定POU4F1,DNASE1L2和WDR72在COAD.中显著上调.
这些基因的表达与结直肠癌患者的预后不佳相关.
建立了一个基于miRNA目标基因的预后特征.

结论:

这些已识别的基因 (POU4F1,DNASE1L2,WDR72) 是COAD的潜在预后生物标志物.
这项研究为COAD的分子病变产生提供了洞察力.
这些发现表明,在COAD中基于RNA的干预措施具有新的治疗目标.

关键词:

在RNA疗法中使用RNA疗法.结肠腺癌是结肠腺癌.这就是miRNAs.预后签名预测签名组织微阵列.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...