相关实验视频
Updated: Jul 15, 2025

08:53
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
2.8K
对于C.的准确和高效的cis-和trans-splicing,需要U6 snRNA m6A的修改. 伊莱根斯的mRNAs
Aykut Shen1, Katarzyna Hencel1,2, Matthew T Parker3,2
1School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
bioRxiv : the preprint server for biology
|September 25, 2023
概括
RNA的修改对于基因表达至关重要. 在C. elegans中,U6 snRNA的m6A修饰对于准确的前mRNAs的cis-和trans-splicing至关重要,确保适当的基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 在RNA生物学,RNA生物学.
背景情况:
- 前信使RNA (前mRNA) 拼接对于真核细胞基因表达至关重要,涉及 cis-splicing 和 trans-splicing 机制.
- 结合体小核RNAs (snRNAs) 和相关蛋白质识别了结合部位序列,而snRNAs上的RNA修改可以影响结合的效率和准确性.
研究的目的:
- 调查m6A修饰在Caenorhabditis elegans (C. elegans) 前mRNA剪接中的U6小核RNA (snRNA) 上的作用.
- 确定U6 snRNA m6A修饰对cis和trans拼接过程的影响.
主要方法:
- 利用C. elegans的遗传方法研究U6 snRNA m6A修饰的影响,特别关注RNA甲基转移酶METT-10.
- 分析了拼接模式,包括5'和3'拼接位的替代拼接,并评估了修改和未修改的U6 snRNA对拼接位的识别.
主要成果:
- 在METT-10的介导下,位于A43位置的U6 snRNA的m6A修饰对于C. elegans的准确和高效的cis-和trans-splicing至关重要.
- 失去U6 snRNA m6A修饰导致替代拼接,特别是在5'拼接位,并损害了较弱的3'跨拼接位的识别.
- U6 snRNA m6A43 和剪接因子 SNRNP27K 合作识别5'剪接部位,其中氨酸位于+4位置.
结论:
- U6 snRNA的m6A修饰是C. elegans中精确的mRNA前拼接的关键调节元件.
- 这种修改在确保cis-和trans-splicing的忠实性方面发挥着重要作用,影响基因表达调节.
相关概念视频
RNA Splicing
56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Alternative RNA Splicing
21.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.2K
Pre-mRNA Processing: RNA Splicing
5.3K
5.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends
9.4K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.4K
Nonsense-mediated mRNA Decay
10.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.7K
RNA Editing
9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

