相关实验视频

Updated: Jul 15, 2025

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

发育性甲状腺暴露破坏了小鼠大脑中MAP激酶和昼夜节律的分子通路

Jennifer H Nguyen¹, Melissa A Curtis¹, Ali S Imami¹

¹Department of Neurosciences, University of Toledo College of Medicine and Life Sciences, Toledo, OH 43614.

bioRxiv : the preprint server for biology

|September 25, 2023

概括

在小鼠中,发育性甲状腺暴露改变了与神经发育障碍 (NDD) 和ADHD相关的脑功能和行为. 这项研究揭示了多模式的生物变化,确定了NDD风险的潜在机制.

科学领域:

神经科学是一个神经科学.
毒理学毒理学毒理学
遗传学遗传学是一种遗传学.

背景情况:

神经发育障碍 (NDD) 缺乏明确的生物标志物,环境因素,如在怀孕期间接触甲状腺杀虫剂,与NDD风险有关.
之前的研究表明,小鼠中低剂量的德尔塔美林暴露会影响与ADHD相关的行为和多巴胺系统.

研究的目的:

通过使用综合的多组学方法,全面调查由发育性甲状腺暴露 (DPE) 引起的小鼠大脑中的生物变化.
确定与环境暴露相关的NDD和ADHD潜在的分子机制.

主要方法:

在怀孕和哺乳期间,小鼠母被暴露于德尔塔美.
后代的大脑使用转录组学,动态组学和多组学集成进行了分析.
采用了分割样本设计和基于垃圾的分析.

主要成果:

转录组分析显示了时钟基因的变化.
基因组分析显示了酶的变化,包括ERK,参与了突触可塑性.
多种组合的整合确定了与MAP激酶级联,亡和突触功能相关的失调的蛋白质-蛋白质相互作用网络.

结论:

发育性甲状腺暴露会诱导与ADHD和NDD相关的多模式大脑生物表型.
该研究强调了神经发育中环境风险因素的新型潜在作用机制.

关键词:

这就是MAP激酶.昼夜节律是指昼夜节律的节奏.发展障碍发展障碍发展障碍动态动态学 (kinomics) 是一种动态学.多种omics集成的整合.翻译学翻译学翻译学翻译学

更多相关视频

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Published on: January 19, 2018

Chronic Sleep Deprivation in Mouse Pups by Means of Gentle Handling

Chronic Sleep Deprivation in Mouse Pups by Means of Gentle Handling

Published on: October 11, 2018

相关概念视频

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...