沉积的足迹让细胞在受限,振荡和探索性迁移之间切换
bioRxiv : the preprint server for biology
|September 25, 2023
概括
细胞迁移受到细胞留下的细胞外基质足迹的影响. 数学建模揭示了这些足迹如何导致细胞以振荡模式移动或探索新环境.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 数学建模的数学建模
背景情况:
- 细胞迁移对于伤口愈合,免疫反应和转移至关重要.
- 细胞与细胞外矩阵 (ECM) 组件相互作用并沉积,产生影响其运动的足迹.
- 之前的实验观察到由于这些足迹而在微型基板上的上皮细胞中发生振荡迁移.
研究的目的:
- 研究足迹沉积和细胞对这些足迹的反应驱动复杂的细胞迁移模式的机制.
- 探索数学建模如何预测和解释新出现的细胞运动行为,包括振荡和探索性现象型.
- 了解足迹相互作用的变化如何导致不同的细胞迁移策略.
主要方法:
- 利用相场模型来模拟细胞爬行动态.
- 整合了细胞极性的一种生化模型,具体包括Rac1通过与沉积足迹的局部接触激活Rac1.
- 模拟细胞行为在一维微型线和二维基板上.
主要成果:
- 证明足迹沉积率和对足迹的细胞反应之间的相互作用可以产生各种运动模式,包括受限,振荡和持久的运动.
- 根据模型参数,预测了从循环运动向基于2D基板的探索性表型的过渡.
- 表明,细胞足迹相互作用的小量的变化可以大大改变迁徙行为,并导致不同的运动现象型.
结论:
- 细胞运动受到足迹沉积和细胞传感机制之间的相互作用的严格调节.
- 足迹沉积或传感的变化可以导致细胞群体内的多样化的运动现象.
- 计算预测与循环和探索性细胞运动的实验观测一致.
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