相关实验视频

Updated: Aug 5, 2026

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

分配:准确地分配多映射的读数,可以在重复时进行调控元件分析

Alexis Morrissey¹, Jeffrey Shi¹, Daniela Q James¹

¹Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA.

bioRxiv : the preprint server for biology

|September 25, 2023

概括

艾洛精确地分析了从重复的DNA区域读取序列的读数,发现了新的基因调控元素. 这种方法增强了可转移元素及其监管作用的表征.

科学领域:

基因组学就是基因组学.
计算生物学计算生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

可转移元素 (TE) 和重复区域含有关键的基因调节元素,包括转录因子结合位.
像ChIP-seq和ATAC-seq这样的短读序列测试在重复中难以表征调控元素,因为丢弃了多映射的读数.
目前的基因组学分析管道错过了重复区域的重要监管事件,因为它不包括多重映射的读数.

结论:

Allo克服了短读测序的局限性,用于分析重复的基因组区域.
这种方法显著扩大了可转移元素中的基因调节元件的发现.
艾洛增强了我们对重复性DNA的监管环境和进化动态的理解.

更多相关视频

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...