长链非编码RNA测序分析揭示了化学诱导的乳腺上皮细胞的分子概况
Mengzhen Wei1, Wenkui Tang1, Danwei Lv1
1Guangxi Key Laboratory of Eye Health, Guangxi Academy of Medical Sciences, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
Frontiers in genetics
|September 25, 2023
概括
长非编码RNAs (lncRNAs) 调节细胞重编程. 这项研究确定了涉及山羊纤维细胞化学诱导到功能性乳腺上皮细胞的差异表达的lncRNAs.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 长非编码RNAs (lncRNAs) 是细胞重编程的关键调节者.
- 之前的研究表明,小分子可以将山羊纤维细胞重新编程成功能性乳腺上皮细胞.
研究的目的:
- 在山羊纤维细胞的化学重编程过程中分析lncRNA表达特征.
- 为了确定参与乳腺上皮细胞诱导的lncRNAs.
主要方法:
- 在重新编程 (0d vs. 8d) 之前和之后对细胞进行了lncRNA-Sequencing (lncRNA-seq).
- 进行了差异性lncRNA表达分析.
- 对目标基因进行了功能丰富分析.
- 实时光量化 (RT-qPCR) 验证了测序结果.
主要成果:
- 总共有3970个候选差异性lncRNAs被检测到 (1170个注释,2800个新型).
- 在重新编程的细胞中,738个lncRNAs被上调和550个被下调.
- 不同表达的 lncRNA 向参与 Wnt,PI3K-Akt,ECM-受体相互作用和 MAPK 信号通路的基因.
- lncRNA和基因表达的变化与改变的细胞表型相关.
结论:
- 这项研究确定了参与纤维细胞乳腺上皮细胞化学诱导的关键lncRNAs.
- 这些发现为了解 lncRNAs 在细胞重编程中的分子机制提供了基础.
- lncRNAs在乳腺发育和细胞命运决定的关键途径中发挥着重要作用.
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