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开发和验证一个QuEChERS-LC-MS/MS方法来确定来自博茨瓦纳的玉米和的多种真菌毒素

Mesha Mbisana¹, Tshepho Rebagamang², Dikabo Mogopodi¹

¹Laboratory of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of Botswana, Gaborone, Botswana.

Frontiers in fungal biology

|September 25, 2023

概括

一种新的QuEChERS-LC-MS/MS方法可以同时检测到玉米和的十种真菌毒素. 这项分析显示,在博茨瓦纳市场的一半样本中,真菌毒素污染,其中两个超出了亚毒素B1限值,突出了需要更严格的监测.

科学领域:

食品安全和分析化学
农业科学和毒理学

背景情况:

干旱和高温等气候条件会增加谷物中的真菌毒素发生.
主要食品 (玉米,) 的污染,由于毒素中毒,对公众健康构成重大风险.
敏感的同时分析方法对于评估菌根毒素暴露至关重要.

研究的目的:

开发和验证一种快速,简单,廉价,有效,坚固和安全的 (QuEChERS) 液体染色学-并联质谱法 (LC-MS/MS) 方法.
允许在玉米和中同时确定十种真菌毒素.
评估来自博茨瓦纳的市场样本中的真菌毒素的流行程度.

主要方法:

优化QuEChERS提取程序以提高回收率和减少矩阵效应.
根据国际监管标准 (EU 2021/808,EC 1881/2006,EC 401/2006) 验证开发的方法.
应用经过验证的方法来分析来自博茨瓦纳的20个市场样本 (10种玉米,10种).

主要成果:

该QuEChERS-LC-MS/MS方法显示了高准确度 (80.77109.83%的恢复) 和精度 (CVs <15%).
获得了优异的线性 (R2 > 0.98) 和低量化极限 (0.5389.28 μg/Kg).
在50%的样本中检测到菌毒素,包括阿弗拉托克辛,米尼辛,T2毒素,HT2毒素和泽拉伦.
两个玉米样本超过了阿弗拉托克辛B1的最大允许水平 (2.55和4.07μg/kg).

结论:

开发的QuEChERS-LC-MS/MS方法适用于在玉米和中同时分析多种真菌毒素.
博茨瓦纳的谷物市场普遍存在菌毒素污染,需要加强监测.
调查结果强调了监测玉米中亚黄素B1的重要性,以确保食品安全.

关键词:

查看 QuEChERS-LC-MS/MS 的时间食品安全食品安全玉米玉米玉米是一种开发方法开发方法的方法.多种真菌毒素多种真菌毒素这就是. . .

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