评估Trichoderma reesei和Aspergillus niger在不同碳来源上生长的细胞内初级代谢特征
Gustavo Pagotto Borin1,2, Juliana Velasco de Castro Oliveira1,2
1Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), São Paulo, Brazil.
Frontiers in fungal biology
|September 25, 2023
概括
在各种碳来源上对Trichoderma reesei和Aspergillus niger的新陈代谢分析揭示了营养同化和应激耐受性的保存机制. 这些发现有助于我们更好地理解如何开发高效的微生物工厂.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 菌类学 菌类学是指菌类学.
背景情况:
- 特里科德尔马·里西和阿斯伯吉卢斯·尼日尔是关键的工业真菌.
- 对这些真菌缺乏综合的奥米克分析.
- 了解它们对不同碳来源的代谢反应对于优化工业应用至关重要.
研究的目的:
- 在不同的碳来源上分析T. reesei和A. niger的细胞内代谢概况.
- 为了研究碳源回对初级新陈代谢的影响.
- 整合代谢数据与转录学,以更深入地了解真菌同化.
主要方法:
- 质子核磁共振 (1H NMR) 谱学用于代谢分析.
- 培养T. reesei RUT-C30和A. niger N402在葡萄糖,乳糖,碳氧甲基纤维素 (CMC) 和蒸汽爆炸的甘包 (SEB) 上.
- 与文献中的现有转录学数据的整合.
主要成果:
- 曼尼托尔,三糖,谷氨酸,谷氨和氨酸在所有碳来源中都很丰富,这表明保存了同化和耐压力机制.
- T. reesei 在乳糖上显示出更高的银河糖中间体,而A. niger在CMC和乳糖上积累了糖醇和4-氨基丁酸盐.
- A. niger 显示了 SEB 的利用率提高,其证据是代谢变化和更高的酶活性.
结论:
- 的初级新陈代谢表现出对不同碳源的保护性反应.
- 特定的代谢物 (如甘,4-氨基酸盐) 在适应某些碳来源方面发挥着重要作用.
- 这项研究为为工业生物生产设计强大的微生物细胞工厂提供了洞察力.
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