在Flavi和Circumdati部分的小NRPS类酶可以选择性地形成替代的pyrazinone代谢物
Matthew D Lebar1, Brian M Mack1, Carol H Carter-Wientjes1
1Food and Feed Safety Research, Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture (USDA), New Orleans, LA, United States.
这项研究揭示了Aspergillus真菌中的AsaC酶可以选择性地产生不同的pyrazinone化合物. 了解这个酶的理解
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 食品安全 食品安全
背景情况:
- 菌产生有害的真菌毒素,如亚毒素和毒素A,影响人类和动物的健康.
- 这些真菌毒素污染了重要的粮食作物,如玉米和大麦.
- 缓解真菌毒素污染是一个重大的全球挑战.
研究的目的:
- 为了研究生物合成基因集群"asa"负责pyrazinone代谢物生产在Aspergillus.
- 了解核心基因产物AsaC在不同Aspergillus部分的功能和选择性.
- 探索潜在的策略,以减轻与真菌毒素有关的健康风险.
主要方法:
- 研究了Aspergillus flavus中的"asa"基因集群.
- 从Aspergillus sclerotiorum中进行的"asaC"正义基因交换到Aspergillus flavus中.
- 从这两个部分克隆了"asaC"的奥托洛格,变成了Saccharomyces cerevisiae.
主要成果:
- "asa"基因群产生阿斯珀吉林酸,这是一个pyrazinone代谢产物.
- 来自AspergillusFlavi部分和Circumdati部分的AsaCortologs选择性地生物合成了不同的pyrazinones:分别是deoxyaspergillic酸和flavacol.
- 尽管ASAC酶仅具有基化-基化-减少酶 (ATR) 域,但它们表现出特定的产物形成.
结论:
- 酶AsaC是选择性生物合成不同的天然产品pyrazinone的关键.
- 了解AsaC的功能和选择性可以为针对真菌毒素的策略提供信息.
- 这项研究有助于改善食品和料安全和人类健康.
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