在DothideomycetousBipolaris maydis中发生的Meiotic沉默
Kenya Tsuji1,2, Yuki Kitade1, Akira Yoshimi1,2
1Laboratory of Terrestrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Frontiers in fungal biology
|September 25, 2023
概括
通过未配对DNA (MSUD) 制造的体沉默会导致双极星疾病中异常的 ascospore 形态. 破坏依赖RNA的RNA聚合酶阻止了这种沉默,证实了MSUD.
科学领域:
- 菌类学 菌类学是指菌类学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 双极虫 (Bipolaris maydis) 是一种有线状虫,它会引起玉米叶病,并且在隔膜突变和野生型菌株之间的交叉中表现出异常的 ascospore 形态.
- 这种现象类似于Neurospora crassa中的"骨主导",与未配对DNA (MSUD) 的介质性沉默有关.
- 在B. maydis中MSUD的存在和功能仍然不清楚.
研究的目的:
- 为了确定B. maydis是否拥有功能MSUD系统,负责ascus主导.
- 研究B. maydis中MSUD的机制及其在线状虫中保存的机制.
主要方法:
- 与携带插入CDC10-septin基因序列的B. maydis突变体进行了同卵性和异卵性交叉试验.
- 在B. maydis.中识别并破坏了依赖RNA的RNA聚合酶基因 (Δrdr1).
- 在Δrdr1菌株和隔膜突变物之间进行了交叉.
主要成果:
- 交叉测试表明,未配对的DNA触发了B. maydis中的ascus主导地位,类似于N. crassa.
- 在B. maydis中RNA依赖RNA聚合酶 (Δrdr1) 的破坏可以在所有交叉中取消ascus主导地位.
- 这些发现表明,MSUD通过非配对基因的RNA沉默来调解,影响B. maydis. 的隔膜基因.
结论:
- 双极星可能拥有功能性的MSUD系统,表明这种转录后调节不仅限于Sordariomycetes.
- 这项研究将MSUD的已知发生范围扩展到Dothideomycetes,这表明有线状虫中存在广泛的保护.
- 七基因被认为是B. maydis.中MSUD诱导的沉默的目标.
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