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小鼠的短期禁食会增加循环纤维细胞生长因子23 (FGF23)

Martina Feger¹, Jana Alber¹, Jörg Strotmann¹

¹Department of Physiology, University of Hohenheim, Stuttgart, Germany.

Acta physiologica (Oxford, England)

|September 25, 2023

概括

禁食和体刺激纤维细胞生长因子23 (FGF23) 的产生. 这一发现具有临床意义,因为FGF23用于监测疾病,禁食可能会影响测试结果.

科学领域:

内分泌学在内分泌学.
代谢过程中的代谢.
骨生物学骨生物学骨生物学

背景情况:

纤维细胞生长因子23 (FGF23) 调节酸盐和维生素D的稳态.
血清FGF23水平与各种疾病结局有关.
众所周知,禁食可以刺激身体的酸生产.

研究的目的:

调查禁食是否通过体生产诱导FGF23的合成.
探索连接禁食,体和FGF23.3的分子机制.

主要方法:

在体外研究中,使用使用UMR106细胞和新生小鼠心室肌细胞 (NRVM) 治疗β-基酸盐.
在体内研究涉及过夜禁食,随意食或 administration-hydroxybutyrate的小鼠.
分析FGF23,体,维生素D代谢物和相关的基因/蛋白质表达,使用ELISA,西式涂抹,qRT-PCR和其他生化分析.

主要成果:

β-基酸盐刺激了UMR106细胞和NRVM中的FGF23产生,以核因子卡帕激活B细胞 (NFκB) 的光链增强剂依赖的方式.
与食小鼠相比,禁食小鼠的血清β-基酸盐和FGF23水平升高,心脏,胰腺和胸腺Fgf23表达增加,脏Cyp24a1表达增强.
禁食导致血清1,25(OH) 2D3和脏Slc34a1和αKlotho (Kl) 表达的降低,但没有显著改变骨Fgf23表达或血清酸盐,,PTH或脏Cyp27b1水平.

结论:

短期禁食和β-基酸盐的使用会增加FGF23的产生.
这些发现表明,禁食状态可以影响FGF23水平,这对疾病监测有潜在的临床影响.
禁食引起的FGF23变化可能会影响酸盐和维生素D的新陈代谢.

关键词:

联邦政府财政基金23 在 NFκBB 的情况下.酸盐酸盐的使用方法维生素D是维生素D的重要组成部分.α 克洛托霍 (Klotho) 是一个

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