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Updated: Jul 15, 2025

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在非状性非小细胞肺癌中采用超快的基于片的下一代测序

Christophe Bontoux¹, Virginie Lespinet-Fabre², Olivier Bordone²

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur, CHU de Nice; Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, Hôpital Pasteur, CHU de Nice; Institut Hospitalo-Universitaire (IHU), RespirERA, Université Côte d'Azur, Hôpital Pasteur, CHU de Nice; FHU OncoAge, Université Côte d'Azur; Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne, Université Côte d'Azur; christophe.bontoux@outlook.com.

Journal of visualized experiments : JoVE

|September 25, 2023

概括

一个超快的下一代测序工作流程准确地识别了非状非小细胞肺癌 (NS-NSCLC) 患者的分子标. 这种快速的分子表征支持及时的向治疗决策,改善患者护理.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
胸部医学胸部医学

背景情况:

非状非小细胞肺癌 (NS-NSCLC) 的分子标数量迅速增加.
分子特征对于指导向疗法和改善晚期NS-NSCLC的存活率至关重要.
瘤发展出抵抗机制,需要持续的分子分析,以寻找新的治疗策略.

研究的目的:

描述一个超快的基于安普利康的下一代测序 (NGS) 工作流程,用于NS-NSCLC诊断.
评估工作流程能够满足建议的短周转时间 (TAT) <10个工作日.
评估工作流程是否适合在常规临床实践中识别可操作的分子标.

主要方法:

在日常日常实践中实现基于amplicon的超快速NGS工作流.
专注于NS-NSCLC患者活检的分子特征.
重点是实现快速的诊断周转时间.

主要成果:

描述的NGS工作流程成功地确定了NS-NSCLC中精密医学的关键分子标.
该系统证明了适当的周转时间,符合临床指南.
工作流程是有效的,尽管挑战与生物检查尺寸下降和不同的组织来源.

结论:

超快速的NGS工作流程是诊断时NS-NSCLC分子分析的可行和高效方法.
这种快速的方法支持在胸部瘤学中及时给予向治疗.
该工作流程解决了在精准医学时代快速,准确的分子表征的需求.

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