酸柏柏林酸盐对Streptococcus mutans在不同pH值下生物膜形成的抑制作用
Yang Zhou1,2, Zhuoying Liu1,2, Jie Wen1,2
1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Microbiology spectrum
|September 25, 2023
概括
柏柏林化水合物 (BH) 有效地抑制Streptococcus mutans生物膜的形成和毒性,在性条件下活性增强. 这种天然化合物在预防牙腐烂方面表现有前途.
科学领域:
- 微生物学 微生物学
- 牙科研究 牙科研究
- 自然产品化学 自然产品化学
背景情况:
- 突变性链球菌 (S. mutans) 是由于其粘附和生物膜形成导致牙损伤的主要原因.
- 柏柏林化水合物 (BH) 是一种植物性化物,具有抗微生物特性.
- BH对S. mutans生物膜的影响,特别是在不同的pH条件下,仍然在很大程度上未被探索.
研究的目的:
- 在不同的pH水平 (酸性,中性,性) 上研究BH对S. mutans的抗菌膜活性.
- 探索pH对BH结构稳定性和生物安全性的影响.
- 阐明BH抑制S. mutans生物膜形成和毒性的潜在机制.
主要方法:
- 检查了BH对S. mutans在pH5,8和中性的抗菌膜活性.
- 在不同的pH值中评估了BH的结构稳定性和对红细胞的毒性.
- 分析了关键的S. mutans基因 (srtA, spaP, gbpC, comX, ldh) 的表达,这些基因参与了粘附,生物膜形成和酸性生产.
主要成果:
- BH显著抑制了S. mutans生物膜的形成,酸的产生和EPS的合成.
- 在酸性条件下,BH的抑制作用降低,但在性条件下增强.
- 在测试的pH值中,BH表现出良好的结构稳定性和低红细胞毒性.
- BH下调基因 (srtA,spaP,gbpC) 对于粘附和生物膜形成至关重要.
- BH降低了comX和ldh的表达,这可能解释了它抑制酸的产生.
结论:
- 柏柏林化水合物是S. mutans生物膜和毒性因子的强有力的抑制剂.
- BH的有效性取决于pH值,在性环境中显示出增加的活性.
- BH提出了一种有前途的治疗药物,用于预防和治疗牙损伤.
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