隔离和描述人类结膜细胞中细胞流体细胞及其细胞外囊
Laura García-Posadas1, Ismael Romero-Castillo1, Kieran Brennan2
1Ocular Surface Group, Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, Spain.
Investigative ophthalmology & visual science
|September 25, 2023
概括
从捐赠组织中成功分离出人类结结膜介质层细胞 (Conj-MSCs). 它们的细胞外囊泡 (EV) 显示出显著的抗氧化活性,保护结膜上皮细胞免受氧化应激.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 介酶体 stromal 细胞 (MSCs) 对于组织修复和再生至关重要.
- 结膜介质细胞 (Conj-MSCs) 在眼皮表面疾病中提供了潜在的治疗应用.
- 来自MSCs的细胞外囊泡 (EVs) 越来越多地因其再生和保护性质而得到认可.
研究的目的:
- 从尸体捐赠组织中分离和培养人类Conj-MSCs.
- 获取和描述由Conj-MSCs.产生的细胞外囊泡 (EVs).
- 评估Conj-MSC衍生的EVs对结膜上皮细胞的治疗效果.
主要方法:
- 从尸体结膜组织中分离和培养人类Conj-MSCs.
- 使用MSC标志物的流动细胞计和体外差异化试验来表征Conj-MSCs.
- 通过差异超离心和使用AFM,DLS和纳米流细胞计的表征,将EVs与Conj-MSCs分离.
- 在体外结膜上皮细胞模型中评估Conj-MSC EV对氧化应激和活力的影响.
主要成果:
- 培养的树皮细胞符合MSC标准,表达关键标记物 (CD90,CD105,CD73,CD44) 并表现出多效.
- 由Conj-MSC衍生的EV被描述为表达常见EV标记物的小圆形囊泡 (CD9,CD63,CD81,CD147).
- 在氧化应激下,Conj-MSC EVs显著降低了结膜上皮细胞中反应性氧物种的产生,与控制EVs和酸相比.
结论:
- 人类Conj-MSCs可以从尸体捐赠组织中成功分离出来.
- Conj-MSCs作为生产细胞外囊泡 (EVs) 的可行来源.
- 由Conj-MSC衍生的EV具有显著的抗氧化特性,对结膜上皮细胞有益.
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