欧蛋白对胃癌细胞系表现出抗癌作用
Mehmed Kürşad Türkdoğan1, Abdurrahim Koçyiğit2, Eray Metin Güler3
1Department of Nutrition and Dietetics, Faculty of Health Sciences, Istanbul Sabahattin Zaim University, Istanbul, Turkey. kursad.turkdogan@izu.edu.tr.
Molecular biology reports
|September 25, 2023
概括
在橄中发现的欧蛋白 (OLE) 在实验室中对胃癌细胞表现出显著的抗癌作用. 这种天然化合物显示出未来作为胃癌治疗的化疗剂的潜力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 欧蛋白 (OLE) 是橄中一种关键的化合物,具有已知的健康益处.
- 胃癌仍然是全球癌症死亡的主要原因,通常与饮食因素有关.
- 需要进一步研究OLE作为抗胃癌抗癌剂的潜力.
研究的目的:
- 在体外 (in vitro) 实验中研究欧蛋白 (OLE) 对胃腺癌 (AGS) 细胞的细胞毒性,基因毒性和亡性作用.
- 评估OLE在调节胃癌细胞中反应性氧物种 (ROS) 生产中的作用.
主要方法:
- 胃腺癌 (AGS) 细胞在24小时内暴露在不同剂量的OLE中.
- 用ATP活力测试测量细胞毒性.
- 基因毒性通过彗星测试,ROS产量通过H2DCF-DA测试,以及通过流式细胞计测试来评估细胞亡.
主要成果:
- OLE显著降低了剂量依赖的AGS细胞活力,最大降低约60%在500μmol/L.
- OLE引发了大量的DNA损伤 (大约2个月). 100%) 和带有亡的亡 (大约. 40%) 在AGS细胞中.
- 细胞内ROS度的增加与细胞活力降低有显著的相关性 (p < 0.050.001).
结论:
- 欧蛋白在体外对胃腺癌细胞表现出显著的抗癌活性.
- OLE证明了对胃癌的保护和治疗潜力.
- 欧蛋白可能成为未来胃癌治疗的新型化疗剂.
相关概念视频
Cancer Prevention
6.2K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
6.2K
Inhibition of Cdk Activity
4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Drugs for Peptic Ulcer Disease: Sucralfate as Mucosal Protective Agents
535
In the intricate landscape of the gastric lumen, excessive acid secretion disrupts the natural defense mechanisms, weakening the mucus-bicarbonate barrier. This vulnerability allows pepsin to infiltrate epithelial cells, digesting mucosal proteins and triggering erosion, leading to ulcer formation.
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
535
Drugs for Peptic Ulcer Disease: Prostaglandin Analogs as Mucosal Protective Agents
478
The gastric mucosa produces prostaglandins E2 (PGE2) and prostacyclin (PGI2), crucial in maintaining gastric health. They exert cytoprotective effects, including increasing bicarbonate secretion, releasing protective mucin, reducing gastric acid output, and preventing harmful vasoconstriction. These effects are mediated through various receptors, such as EP1, EP2, EP3, and EP4.
Non-steroidal anti-inflammatory drugs (NSAIDs) can induce peptic ulcers by inhibiting cyclooxygenase, decreasing...
Non-steroidal anti-inflammatory drugs (NSAIDs) can induce peptic ulcers by inhibiting cyclooxygenase, decreasing...
478
Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
503
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
503


