酸脱酶超级家族结构基因组学蛋白质的功能注释
Lakindu S Pathira Kankanamge1, Lydia A Ruffner1, Mong Mary Touch1
1Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, U.S.A.
The Biochemical journal
|September 25, 2023
概括
这项研究描述了酸脱酶 (HAD) 超级家族中的新型酶. 研究人员在几个蛋白质中确定并验证了脱酶和糖酸酶的活动,揭示了基质乱交.
科学领域:
- 生物化学 生化学
- 酶学 是一种酶学.
- 结构基因组学 结构基因组学
背景情况:
- 酸脱酶 (Haloacid Dehalogenase,简称HAD) 超级家族包括各种各样的酶,其功能尚不清楚.
- 成员可以作为糖酸酶,酸脱酸酶,ATPases等.
- 结构基因组学倡议旨在表征功能不明的蛋白质.
研究的目的:
- 预测和实验验证HAD超级家族蛋白质的功能,特别是结构基因组学项目中的蛋白质.
- 为了确定这个大型蛋白质家族内的特定酶活性.
主要方法:
- 基于化学和静电性质的蛋白质功能的计算预测.
- 直接进行生化分析,以测试预测的酶活性.
- 对9种代表性HAD蛋白和21种结构基因组蛋白进行分析.
主要成果:
- 一种类似于脱基酶的酶 (RSc1362) 已被证实具有脱基酶活性.
- 四种蛋白质被描述为具有不同基质特异性的糖酸酶.
- 确定的活动包括三糖-6-酸盐酸酶,果糖-6-酸盐酸酶和糖糖-6-酸盐酸酶.
- 在几个已识别的糖酸酶中观察到基质乱交.
结论:
- 以前未被注释的HAD超级家族蛋白质的功能性特征.
- 用于酶功能的预测计算方法的验证.
- 在HAD超级家族中扩展已知的酶谱,突出显示糖酸酶和脱酸酶活动.
更多相关视频
05:08Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
Published on: July 8, 2025
36
07:08Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
Published on: July 14, 2015
7.3K
相关概念视频
Gene Families
8.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
8.9K
Protein Families
15.4K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key...
15.4K
Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Globular and Fibrous Proteins
43.8K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
43.8K
Structural Protein Function
27.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.8K
Prokaryotic Gene Structure and Organization
68
Prokaryotic genomes exhibit a streamlined organization of coding and non-coding regions essential for gene expression and protein synthesis. While coding regions contain the genetic instructions for proteins or functional RNAs, non-coding regions regulate the precise transcription and translation of these genes.Coding Regions: Proteins and RNAsThe primary coding regions, known as structural genes, include sequences transcribed into messenger RNA (mRNA) and ultimately translated into...
68
