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进化增强的三步毛细体电泳-SELEX用于外体囊泡的阿帕特默选择

Liping Zhao¹, Ge Yang², Chao Zhu³

¹Institute of Quality Standard and Testing Technology of BAAFS, Agricultural Product Quality and Safety Risk Assessment Laboratory of the Department of Agriculture, Beijing 100097, China; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, China.

|September 25, 2023

概括

研究人员开发了一种新的毛细管电泳-SELEX策略,以有效地选择自然杀手 (NK) 细胞外体的体. 这种方法可以快速分离特定的体,这对于外体检测和治疗应用至关重要.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
纳米技术纳米技术

背景情况:

外体在细胞间通信中至关重要,作为疾病生物标志物和药物载体.
高效的异构体隔离和检测需要开发特定的识别元素.
由于它们的高度亲和力和特异性,aptamers是外体识别的有希望的候选者.

研究的目的:

开发一种新有效的策略来对抗外体囊泡的体选择.
为了证明毛细管电泳-SELEX (CE-SELEX) 对于向外体的体发现的实用性.
为了获得天然杀手 (NK) 细胞外体的高亲和度和高特异性体.

主要方法:

基于CE-SELEX的三步进化增强策略被提议用于aptamer选择.
自然杀手 (NK) 细胞外体被用作aptamer选择的模型标.
选择过程涉及五轮的CE选择,具有不同的进化步骤:序列融合,亲和进化和负选择.

主要成果:

使用提出的CE-SELEX策略,成功选择了针对NK细胞外体的aptamers.
选择的体体表现出良好的亲和力,其K_D值为27.6nM.
这些体对NK细胞外体具有很好的特异性.

结论:

开发的CE-SELEX策略提供了一种有效的方法,用于对外体囊泡进行aptamer选择.
这种新的方法为开发基于外体的诊断和治疗的识别元素铺平了道路.
选择的阿普坦基具有在外体检测和向药物输送方面的应用潜力.

关键词:

亚普特美尔 (Aptamer) 是一种药物.在CE-SELEXEX中使用.在 NK 外基因组中,选择策略选择策略

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