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草甘对E. сolioli生物膜的形成,突变和应激反应的影响
Ivan Sazykin1, Ekaterina Naumova1, Tatiana Azhogina1
1Southern Federal University, 194/2 Stachki Avenue, Rostov-on-Don 344090, Russian Federation.
Journal of hazardous materials
|September 25, 2023
概括
草甘除草剂具有高毒性,并破坏细菌生物膜,影响土壤微生物群落. 它还抑制或增强大肠杆菌的突变发生,对自然微生物群产生潜在的负面影响.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 草甘是一种广泛使用的除草剂,具有不清楚的基因毒性和致变性.
- 它对对土壤微生物群落至关重要的细菌生物膜的影响尚不清楚.
研究的目的:
- 使用大肠杆菌模型评估草甘的毒性,致变性和对细菌生物膜形成和活性的影响.
- 研究草甘对细胞损伤,氧化应激和蛋白质损伤的影响.
主要方法:
- 利用发光全细胞细菌lux生物传感器来评估细胞损伤.
- 使用瑞芬素突变物方法评估突变发生的变化.
- 对细菌生物膜生物质,生理活动和细胞活力的量化影响.
主要成果:
- 草甘表现出高整体毒性,增加氧化应激和E. coli中的蛋白质损伤.
- 所有测试度都抑制了大肠杆菌生物膜的形成和代谢活动.
- 草甘在较高度下抑制了突变发生,但在较低度 (0.0067 g/L) 下增强了6倍.
结论:
- 草甘对微生物具有显著的毒性风险,并破坏重要的细菌生物膜.
- 对突变发生的双重效应 (抑制和增强) 需要进一步研究.
- 草甘对土壤微生物的影响可能导致不利的生态后果.
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