在环境微生物元基因组学研究中寻求标准化的参考材料的建立
1Department of Civil Engineering, Environmental Microbiome Engineering and Biotechnology Laboratory, Centre for Environmental Engineering Research, The University of Hong Kong, Hong Kong, China.
Water research
|September 25, 2023
概括
由于技术因素,特别是DNA提取套件,元基因组测序工作流程的差异很大. 开发复杂的环境参考材料对于确保可重复和准确的微生物组研究至关重要.
科学领域:
- 环境科学环境科学
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 大基因组测序推进了环境微生物组研究.
- 多样化的方法引入工作流异质性和可变性.
- 为了可靠的测序输出,需要标准化的基准测试.
研究的目的:
- 从常见的元基因组工作流变量量化微生物分析变异.
- 开发和验证用于基准测试的复杂环境参考材料.
- 提高环境元基因组学的可复制性和可比性.
主要方法:
- 单变量控制方法用于评估6个技术变量.
- 活性污泥的测序和一个经过认证的模拟社区.
- 基于模拟社区资料重建的测试排名.
主要成果:
- DNA 隔离和图书馆构建显示出最小的变化.
- DNA提取套件和测序运行批量导致了最高的变异性.
- 确定了一种可重现的测定方法,并描述了参考材料的微生物概况.
结论:
- 技术变化,特别是来自DNA提取的技术变化,会影响转基因组学结果.
- 复杂的环境参考材料可以对协议进行基准测试,并提高数据可靠性.
- 采用这种参考材料将促进准确和可比的环境微生物组研究.
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