通过PbPDCB16介导的质沉积会影响等离子体的阻塞,并减少梨果中的化
Wen Li1, Kaili Yuan1, Mei Ren1
1College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Plant science : an international journal of experimental plant biology
|September 25, 2023
概括
梨PD胆结合蛋白 (PDCB) 基因的过度表达,如PbPDCB16,促进胆的积累. 这减少了红素含量和细胞间通信,为改善梨果质量提供了洞察力.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 石细胞,一种化细胞,显著影响梨果的质量和经济价值.
- 焦糖在调节等离子体 (PD) 透性的作用及其对细胞化的影响仍然不清楚.
研究的目的:
- 为了对梨中的PD结蛋白 (PDCB) 基因家族进行全基因组分析.
- 调查PDCB基因,特别是PbPDCB16对质沉积,素积累和细胞间通信的影响.
主要方法:
- 在梨子基因组中对25个已识别的PbPDCB基因进行全基因组分析.
- 定量实时PCR (qRT-PCR) 用于分析基因表达.
- 在梨果和中进行过渡和稳定的表达研究.
- 传输电子显微镜 (TEM) 在转基因Arabidopsis中.
主要成果:
- 25个PbPDCB基因被确定并分为四个进化分支.
- PbPDCB16表达与素积累有负相关性.
- 过度表达PbPDCB16导致胆的增加和红素含量的降低.
- 转基因阿拉比多普西斯表现出较慢的生长,更薄的细胞壁,更少的PD和阻断的细胞间纤维.
结论:
- 过度表达PbPDCB16促进了PD中的胆积累,损害了细胞间连接和通信.
- 这项研究为通过粉沉积来降低素含量提供了理论依据,影响梨中的石细胞形成.
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