野生石灰的psyllid Leuronota fagarae Burckhardt (Hemiptera: Psylloidea) 像皮科纳的病毒全基因组注释和分类
Douglas S Stuehler1, Wayne B Hunter2, Yisel Carrillo-Tarazona3
1ORISE Participant, DOE/USDA, ARS, Fort Pierce, FL 34945, USA; USDA, ARS, 2001 South Rock Road, Fort Pierce, FL 34945, USA.
Journal of invertebrate pathology
|September 25, 2023
概括
在佛罗里达州的野生灰类植物中发现了一种新的像皮科纳病毒,Leuronota fagarae picorna-like virus (LfPLV-FL). 这一发现有助于对昆虫病毒和入侵性亚洲类植物的研究.
科学领域:
- 病毒学 病毒学
- 昆虫学 昆虫学是一门学科.
- 生物信息学是一种生物信息学.
背景情况:
- 皮科纳病毒是一种多样化的RNA病毒群,感染各种宿主.
- 野生石灰 (WLP),Leuronota fagarae是一种已知的昆虫载体.
- 亚洲类植物 (ACP),Diaphorina citri,是佛罗里达州的主要农业害虫.
研究的目的:
- 从WLP.分类和描述一个新分离的皮科纳样病毒.
- 研究新型病毒与其他感染昆虫的病毒的进化关系.
- 探索在管理昆虫害虫和相关疾病方面的潜在应用.
主要方法:
- 从WLP中分离和测序病毒RNA.
- 病毒基因组和多蛋白质的生物信息分析.
- 与已知的像皮科纳病毒进行比较序列分析.
主要成果:
- 在WLP.中发现了一种新的像皮科纳病毒LfPLV-FL.
- LfPLV-FL与在ACP发现的病毒具有同质性.
- 序列分析表明,与感染昆虫的iflavirus存在进化联系.
- 病毒基因组有10006个核酸,编码2942个氨基酸聚蛋白.
结论:
- LfPLV-FL代表了一种新的像皮科纳病毒的病毒物种,与iflavirus有着进化联系.
- 这一发现为病毒宿主特异性和传播提供了洞察力.
- 进一步的研究可以探索LfPLV-FL作为控制ACP和黄龙平 (HLB) 的载体.
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