重组内酶IdeS和IdeZ及其潜在应用
Irina S Boksha1,2, Vladimir G Lunin3, Tatyana A Danilova3
1Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russia. boksha_irina@mail.ru.
Biochemistry. Biokhimiia
|September 25, 2023
概括
链球菌内酶IdeS和IdeZ,可以分裂IgG,已成功克隆,表达和净化. 重组IdeZ显示出用于诊断马匹杀和其他感染的前景.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 末酶IdeS和IdeZ是链球菌毒性因子,可以分裂IgG重链.
- 这些酶在医学,兽医科学和生物技术中具有潜在的应用.
研究的目的:
- 在大肠杆菌中克隆和表达编码IdeS和IdeZ的基因.
- 为了净化和表征复合内酶.
- 评估IdeZ在兽医中的诊断潜力.
主要方法:
- 在大肠杆菌中基因克隆和异质表达.
- 引入6His标签用于净化.
- 使用金属 afinity 染色法进行净化.
- 使用聚烯胺凝电泳的活性和特异性测定.
- 在马类感染的免疫学分析中应用复合IdeZ.
主要成果:
- 成功克隆并表达了Streptococcus pyogenes中的IdeS和Streptococcus zooepidemicus中的IdeZ.
- 净化了IdeS和IdeZ,使它们看上去具有同质性.
- 证明了对人类和动物IgG的酶活性.
- 已确认对人类IgG的特异性.
- 使用重组IdeZ用于诊断马匹杀和确定抗体标位.
结论:
- 重组IdeS和IdeZ是活性和特定的IgG降解性内酶.
- IdeZ是诊断由Streptococcus equi和Streptococcus zooepidemicus引起的感染的一个有价值的工具.
- 这些酶在医学,生物技术和兽医诊断领域具有很大的应用潜力.
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