植物激素通过UPR激活影响人类皮肤纤维细胞的分化状态
Ekaterina P Turishcheva1, Mariya S Vildanova2, Polina A Vishnyakova3,4
1Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. kitten-caterina@yandex.ru.
Biochemistry. Biokhimiia
|September 25, 2023
概括
植物激素如酸 (ABA) 和酸 (GA) 调节纤维细胞活性. ABA促进细胞外基质的产生和肌纤维细胞分化,而GA具有对比的效果.
科学领域:
- 生物化学 生化学
- 细胞生物学 细胞生物学
- 皮肤病学 皮肤病学
背景情况:
- 纤维细胞分化和分泌活动在生物医学研究中至关重要.
- 展开的蛋白质反应 (UPR) 与纤维细胞分化有关.
研究的目的:
- 研究植物激素对人类皮肤纤维细胞分泌活动和分化的影响.
- 探索植物激素在调节纤维细胞中展开的蛋白质反应 (UPR) 中的作用.
主要方法:
- 培养的人类皮肤纤维细胞被用酸 (ABA) 和吉伯瑞利酸 (GA) 处理.
- 分析了UPR标记物 (GRP78,ATF4,CHOP) 的基因表达.
- 评估了分泌活性,包括前原I,纤维内素和总蛋白质生产.
- 肌纤维细胞分化通过α-SMA表达和细胞数量来评估.
主要成果:
- ABA上调调节了GRP78和ATF4的表达,增加了公原I和纤维内素分泌,并促进了α-SMA合成,从而产生了更多的肌纤维细胞.
- GA提高了CHOP表达的调节,增加了纤维内素分泌,但降低了procollagen I合成和总ECM原蛋白生产.
- 此外,GA还降低了α-SMA合成的调节,并减少了肌纤维细胞的数量.
结论:
- 植物激素,特别是ABA和GA,显著调节纤维细胞生物合成活动和分化状态.
- ABA促进了亲纤维化表型,而GA似乎抑制了它,这表明在组织重塑中有不同的作用.
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