相关实验视频
Updated: Jul 15, 2025

09:16
Assessment of Kidney Function in Mouse Models of Glomerular Disease
Published on: June 30, 2018
17.7K
从模式到蛋白质:质谱在淋巴细胞疾病中成长
Sanjeev Sethi1, Jason D Theis1, Lilian M P Palma2
1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota.
Journal of the American Society of Nephrology : JASN
|September 26, 2023
概括
激光捕获微解剖和质谱学 (LCM/MS) 通过识别蛋白质标来推进淋巴细胞疾病的诊断. 这种技术对于诊断氨基粉症至关重要,并有可能用于其他脏疾病,如膜性脏病.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 蛋白质组学是指蛋白质组学.
- 生物标志物发现发现
背景情况:
- 激光捕获微解剖和质谱法 (LCM/MS) 是一种从脏活检中解剖和分析质蛋白质的技术.
- 慢性脑膜炎/多发性脑膜炎在粉样性粉症,纤维性脑膜炎和膜性脏病 (MN) 中识别新标的过程中发挥了重要作用.
- 它还证实了补充蛋白在C3球病变中的作用,是诊断和类型化粉样性病的黄金标准.
研究的目的:
- 审查LCM/MS在各种质细胞疾病中的实用性,不仅仅是粉症,重点是MN,沉积疾病和补充通路障碍.
- 倡导更广泛的临床采用LCM/MS,特别是针对MN的抗原检测.
- 讨论将LCM/MS从研究工具转化为常规临床试验的挑战.
主要方法:
- 使用激光捕获微解剖,从嵌入氨酸的活检组织中剖析质细胞.
- 切割的球蛋白质的蛋白质分解消化使用素.
- 通过质谱学识别和半量化膜蛋白质.
主要成果:
- LCM/MS已经成功地确定了新型蛋白质标和生物标志物,这些生物标志物存在于诸如粉样化症,纤维状GN和MN等疾病中.
- 该技术已经验证了补充蛋白在球体疾病 (如C3球体病) 中的参与.
- LCM/MS被确立为amyloidosis诊断和类型的黄金标准.
结论:
- LCM/MS是一种强大的工具,在诊断和了解质细胞疾病方面应用范围不断扩大.
- 建议常规临床使用LCM/MS,特别是在MN中检测抗原.
- 未来的应用包括阐明脏疾病中补体激活,炎症和纤维化的途径.
相关概念视频
Nephrotic Syndrome II : Assessment and Medical Management
8
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
8
Renal Corpuscle
2.5K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
2.5K
Mass Spectrometry: Complex Analysis
818
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
818
Nephrotic Syndrome I : Introduction
9
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
9
Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K

