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双重生物强化的cisgenic番茄含有增加的黄酸和分支链氨基酸含量
Marta Vazquez-Vilar1, Asun Fernandez-Del-Carmen1, Victor Garcia-Carpintero1
1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universitat Politècnica de Valéncia, Valencia, Spain.
Plant biotechnology journal
|September 26, 2023
概括
西斯基尼番茄是为了增加有益的黄和分支链氨基酸 (BCAA) 而设计的,这为改善心血管健康和预防肌肉损失提供了潜在的策略.
科学领域:
- 植物生物技术 植物生物技术
- 营养科学 营养科学
- 代谢工程是代谢工程.
背景情况:
- 黄类化合物支持心血管健康,而分支链氨基酸 (BCAA) 对于预防肌肉损失至关重要,特别是在纯素饮食中.
- 许多植物性食品缺乏足够的BCAA或广泛的黄酸含量,造成营养差距.
- 代谢工程作物提供了一个潜在的解决方案,以提高营养成分.
研究的目的:
- 开发生物强化的cisgenic番茄,其含有黄酸和BCAA.
- 评估使用内源性番茄遗传元素用于代谢增强的可行性.
- 建立一个评估这些增强番茄安全性和有效性的基础.
主要方法:
- 使用番茄衍生的DNA元素生成 cisgenic 番茄.
- 引入一种抗除草剂的乙酸盐合成酶 (mSlALS) 基因,用于BCAA的丰富和选择.
- 一种类似MYB12的转录因子 (SlMYB12) 的水果特异表达,以增加黄酸含量.
- 代谢学和转录学分析以描述工程番茄的特征.
主要成果:
- 在Cisgenic番茄中,BCAA的含量显著增加:白氨酸 (21倍),氨酸 (9倍) 和异氨酸 (3倍).
- 与抗氧化剂黄类药物同时进行生物强化,包括kaempferol (64倍) 和quercetin (45倍).
- 与野生类型番茄相比,新陈代谢和转录组概况显示出明显的差异.
结论:
- 代谢工程的cisgenic番茄可以成功地与BCAA和黄酸盐进行丰富.
- 这种方法为提高植物性食品的营养价值提供了一种新的策略.
- 对于这些生物强化水果的人类消费,需要进一步进行安全评估.
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