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微流体单细胞迁移芯片揭示了细胞外矩阵对细胞运动影响的洞察力
Mengli Zhou1,2,3, Yushu Ma1,2, Edwin C Rock4
1UPMC Hillman Cancer Center, University of Pittsburgh, 5115 Centre Ave, Pittsburgh, PA 15232, USA. cheny25@upmc.edu.
Lab on a chip
|September 26, 2023
概括
这项研究引入了微流体芯片,用于高通量细胞迁移分析. 原涂层显著增强三阴性乳腺癌细胞的运动,揭示了运动性相关疾病的新治疗点.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 细胞迁移对健康和疾病至关重要,但传统的测试与单细胞跟踪作斗争.
- 了解细胞外矩阵 (ECM) 对细胞迁移的影响对于疾病治疗至关重要.
研究的目的:
- 开发一种高通量微流体芯片,用于精确的大规模细胞迁移监测.
- 调查各种ECM对三阴性乳腺癌细胞迁移和复合效应的影响.
主要方法:
- 使用了一种新型的微流体芯片,整合了机器人和计算机视觉,用于跟踪3200个单独的细胞.
- 评估了10种不同的ECM涂层和87种化合物的细胞迁移.
- 在ECM涂层基板上的细胞中执行了ScRNA-Seq并观察了actin动态.
主要成果:
- 原IV,III和I涂层显著增强了细胞迁移.
- 对细胞迁移的复合效应在不同的ECM基板上有明显的差异.
- ECM涂层诱导了上皮层-介质酶过渡 (EMT) 和高调特定基因,特别是与原IV.
结论:
- 开发的微流体平台提供了高分辨率,高通量细胞迁移分析.
- ECM的组成极大地影响细胞迁移,影响治疗化合物的疗效.
- 针对ECM特定的途径,如由原IV诱导的途径,对疾病治疗有希望.
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