对几种HPLC方法进行维生素C分析的比较
1R.J. Reynolds Tobacco Co., Winston-Salem, North Carolina, USA.
Biomedical chromatography : BMC
|September 26, 2023
概括
本研究介绍了一种使用乙醇稳定酸 (维生素C) 样本进行分析的方法. 它比较了三种HPLC方法,发现LC-MS/MS非常敏感,但对于常见的食品产品并不总是必要.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 亚酸 (维生素C) 是重要的,但容易氧化,使其精确的分析复杂化.
- 由于氧化而导致的样品和标准不稳定性是 Askorbic 酸量化的一个常见挑战.
研究的目的:
- 用mercaptoethanol来描述保护 Askorbic 酸免受氧化的一种程序.
- 为了比较三种高性能液体染色学 (HPLC) 方法用于酸分析的性能.
- 为了评估不同的检测技术 (UV和MS/MS) 和逆相列.
主要方法:
- 开发了一种使用mercaptoethanol的甲酸稳定方法.
- 对比了三个HPLC方法:一个LC-UV和两个LC-MS/MS.
- 评估了两个逆相列进行分离.
- 对各种度范围 (ng/ml到μg/ml) 的校准方法.
- 在能量饮料和中分析维生素C的应用方法.
主要成果:
- 默卡普托乙醇有效地保护了 Askorbic 酸样本免受氧化.
- 通过LC-UV方法量化阿斯科布酸从3.9μg/ml到500μg/ml.
- LC-MS/MS方法提供了更高的灵敏度 (降至0.1 ng/ml),适用于微量分析.
- 在ascorbic酸水解过程中,被认为2,3-diketogulonic酸的形成不太可能.
结论:
- 默卡波乙醇辅助方法增强了用于HPLC分析的 Askorbic 酸稳定性.
- 虽然LC-MS/MS提供了更高的灵敏度,但LC-UV足以分析许多食品中的维生素C.
- 这些发现澄清了甲酸氧化途径,驳斥了水解后的2,3-二基古龙酸形成的意义.
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