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在西班牙探索肉毒神经毒素BoNT/B2的遗传背景

Sylvia Valdezate¹, Gema Carrasco¹, María J Medina¹

¹Reference and Research Laboratory for Taxonomy, National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda , Madrid, Spain.

Microbiology spectrum

|September 26, 2023

概括

在西班牙对Clostridium botulinum菌株的基因分析揭示了各种各样的神经毒素BoNT/B2克隆,对食物传播和婴儿肉毒症病例负责,排除了单一的共同来源.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
流行病学流行病学

背景情况:

肉毒症是一种严重的麻疾病,由肉毒神经毒素 (BoNTs) 引起.
神经毒素BoNT/B2是西班牙人类肉毒的重要原因.
了解致病原体的遗传多样性对于追踪疫情至关重要.

研究的目的:

研究在西班牙生产BoNT/B2的Clostridium botulinum菌株的遗传多样性和遗传学关系.
要确定在西班牙导致肉毒的BoNT/B2产生菌株是否是克隆的.
建立一种方法来追踪西班牙的肉毒病事件.

主要方法:

毒素基因 (邦特亚型) 的测序,鞭毛蛋白基因 (flaVR) 的可变区域,以及七基因多部位序列类型 (ST).
从食品传播和婴儿肉毒症病例 (2010-2022) 中分析了37种BoNT阳性Clostridium botulinum培养物.
遗传学分析和歧视指数 (HGDI) 的计算.

主要成果:

确定了8个不同的bont/b2等位基因,其中一个等位基因占主导 (87.5%).
检测到四种已知的flaVR类型和一种新型类型 (flaVR-15).
记录了16种序列类型 (ST),包括7种新的ST和5种具有新型等位基因组合的新ST,这表明了显著的遗传多样性.

结论:

对bont/b2,flaVR和ST的综合分析提供了一种可靠的方法来追踪西班牙的肉毒事件.
产生BoNT/B2的各种各样的Clostridium botulinum克隆是西班牙人类肉毒症的原因.
观察到的遗传多样性排除了这些肉毒病例的共同来源.

关键词:

在 BoNT BoNT 中使用.在 BoNT/B2 中使用.克洛斯特里迪亚姆肉毒 (Clostridium botulinum) 是一种植物.这里是ST ST ST.西班牙西班牙西班牙西班牙西班牙波特/B2B2 的时间肉毒主义 (botulism) 是一种疾病.这就是为什么FlaVRVR.通过食物传播的食物.婴儿婴儿婴儿婴儿婴儿婴儿婴儿神经毒素是一种神经毒素.

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