来自捐赠者和接受者的病毒特异性TRM细胞存在于人类移植中
Daphne M Hullegie-Peelen1, Hector Tejeda Mora1, Dennis A Hesselink1
1Department of Internal Medicine, Nephrology and Transplantation, Erasmus University Medical Center (Erasmus MC) Transplant Institute.
JCI insight
|September 26, 2023
概括
脏移植中的组织居住淋巴细胞 (TRL) 包括多样化的细胞,捐赠者和接受者TRL都可能保护人免受病毒感染. 这些细胞具有相似的基因表达,并向常见的病毒.
科学领域:
- 免疫学 免疫学 免疫学
- 移植免疫学 移植免疫学
- 病毒学 病毒学
背景情况:
- 组织居住性淋巴细胞 (TRLs) 对于外围组织的局部免疫至关重要.
- 它们在免疫抑制下移植器官中的作用在很大程度上是未知的.
- 在移植中了解TRL对于管理移植后感染至关重要.
研究的目的:
- 在人体移植切除术中描述TRL区间.
- 调查TRL在移植脏中的抗病毒免疫力中的潜在作用.
- 在移植中确定TRL的来源 (捐赠者与接受者).
主要方法:
- 对人类移植切除样本的分析.
- 使用居住标记 (CD69,CD103,CD49a) 来识别TRL种群.
- 配对单细胞转录组和T细胞受体 (TCR) 测序.
- 病毒德克斯特拉默染色以评估T细胞特异性.
主要成果:
- 移植TRL包括多种先天性,先天性类型和适应性种群.
- 在所有TRL子集中,在43%的移植中观察到捐赠者-接受者细胞合体.
- 捐赠者和接受者的组织内存T (TRM) 细胞表现出相似的转录组形状和共享的病毒特异性TCR克隆型.
- TRM细胞向了常见的病毒,如CMV,EBV,BK多种病毒和A型流感病毒.
结论:
- 在移植中存在多样化的TRL群体.
- 捐赠者和接受者TRM细胞都存在于移植中.
- 这些TRM细胞可能有助于移植器官的局部抗病毒免疫力.
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