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TOBF1通过调节多能性基因的替代拼接来调节小鼠胚胎干细胞的命运

Meghali Aich¹, Asgar Hussain Ansari¹, Li Ding²

¹CSIR- Institute of Genomics and Integrative Biology, New Delhi 110025, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

|September 26, 2023

概括

长非编码RNA相关蛋白TOBF1调节小鼠胚胎干细胞 (ESC) 中的替代拼接 (AS). 这一规定对于通过控制多能性相关基因的表达来维持ESC身份至关重要.

科学领域:

干细胞生物学干细胞生物学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

胚胎干细胞 (ESC) 分化成各种细胞类型.
转录因子和染色质修饰剂是已知的ESCs中的调节剂.
替代拼接 (AS) 在调节ESC基因表达中的作用仍未得到充分研究.

研究的目的:

研究长非编码RNA (lncRNA) 相关蛋白TOBF1在调节小鼠ESC中的替代拼接 (AS) 中的作用.
了解TOBF1如何为维持ESC身份做出贡献.

主要方法:

在老鼠ESC中,CRISPR-Cas9对TOBF1进行了淘汰.
RNA测序用于分析基因表达和AS的全球变化.
染色体免疫沉测序 (ChIP-seq) 用于确定TOBF1结合位点.
分析了OCT-SOX结合因子及其分布.

主要成果:

TOBF1调节了转录的AS,这对于保持鼠标ESC身份至关重要.
淘汰TOBF1影响了氨酸/氨酸拼接因子1 (SRSF1) 的AS.
改变的SRSF1 AS导致下游多能性基因的拼接和表达的全球变化.
确定TOBF1占用率,OCT-SOX动机和AS事件汇聚的特定核领土.

结论:

TOBF1在调节小鼠ESC中的替代拼接方面发挥着重要作用.
以TOBF1为媒介的AS对于维持ESC多能性和身份至关重要.
该研究确定了涉及lncRNA相关蛋白质在干细胞维护中的新型调节机制.

关键词:

科普:干细胞研究这就是CRISPR干扰的原因.海外国家和地区SOX.这就是"五旬节".在SRSF1中,我们可以使用SRSF1.在 TOBF1 中,TOBF1 是 TOBF1.替代性拼接是一种替代性的拼接.mESCs是什么意思多能性的多能性.

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