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可靠的多重生成聚合诱导多能干细胞

Molly Smullen¹, Meagan N Olson¹, Julia M Reichert²

¹Department of Neurology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA; Program in Bioinformatics and Integrative Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA; NeuroNexus Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.

Cell reports methods

|September 26, 2023

概括

我们从聚合细胞 (iPPC) 开发了诱导多能性,以有效地将许多体细胞重新编程成诱导多能干细胞 (iPSC). 这种可扩展的方法允许对体外表型和重编程能力进行多重分析.

科学领域:

干细胞生物学干细胞生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

将体细胞重新编程成诱导多能干细胞 (iPSC) 对于体外研究至关重要.
目前的方法缺乏高吞吐量和可扩展性来分析多样化的细胞群.

研究的目的:

开发一种可扩展和高效的方法,用于将组合体细胞重新编程成iPSC.
为了使单个重编程效率和体外表型的多重分析.

主要方法:

开发了从聚合细胞 (iPPC) 程序中诱导多能性.
利用一个解卷算法与单核酸多态 (SNPs) 的聚合测序来确定个体捐赠者比例.
同时重新编程超过一百个捐赠淋巴细胞细胞系 (LCLs).

主要成果:

通过iPPC,可以有效,可扩展和可靠地将聚合的LCL重编程为iPSC.
使用SNP解卷方法,准确估计个人捐赠者比例在聚合的iPSC中的比例.
在实验中保持一致的个人捐赠者重编程能力,免疫球蛋白前体基因表达的潜在影响.
成功地将聚合的iPSCs分化为大脑器官.

结论:

iPPC提供了一个强大的多重组框架,用于生成聚合的iPSC库.
促进了体外表型和供体特异性重编程效率的大规模研究.
通过使用多样化的iPSC集合,实现了先进的下游研究应用.

关键词:

CP: 干细胞是一种干细胞.生物信息学是一种生物信息学.解体解体是一种解体.不同化的差异化差异化.流动细胞计量是流动细胞计量的方法.基因组学就是基因组学.这些是iPSCs.一个多重复合并的多重复合并.有机生物有机物重编程是重新编程.测序的测序是指测序的测序.

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