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在同步多色单分子定位显微镜中评估交叉声谱

Karoline Friedl¹, Adrien Mau², Fanny Boroni-Rueda³

¹Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France; Abbelight, 191 Avenue Aristide Briand, 94230 Cachan, France.

Cell reports methods

|September 26, 2023

概括

这项研究引入了更快的多色单分子局部化显微镜 (SMLM) 方法. 在dSTORM (SD-dSTORM) 中进行光谱解为先进的多色SMLM调查提供了强大的选择.

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
纳米技术纳米技术

背景情况:

单分子定位显微镜 (SMLM) 实现了纳米尺度的分辨率.
像STORM和DNA-PAINT这样的技术是已建立的SMLM方法.
更快的多色SMLM对于复杂的生物成像至关重要.

研究的目的:

实施和评估两种新的方法,以实现更快的多色SMLM.
评估dSTORM (SD-dSTORM) 中光谱脱杂的交叉声和生物相关性.
将这些方法扩展到3D和三色成像.

主要方法:

开发了同时进行的双色DNA-PAINT (S2C-DNA-PAINT) 和光谱除混合的dSTORM (SD-dSTORM).
在两台摄像机之间分割发射的光,用于同时成像.
通过使用DNA纳米制造器和标记细胞评估SD-dSTORM交叉声.
评估了交叉语音对检测亚衍射模式的影响.

主要成果:

S2C-DNA-PAINT 作为低交叉声的参考.
在各种样本类型中评估了SD-dSTORM交叉通话.
评估了对生物模式的交叉影响.
证明了成功扩展到3D采集和三色成像.

结论:

光谱解混 (SD-dSTORM) 是一种可行的多功能方法,用于多色SMLM.
这些进步使纳米尺度成像中的更强大的调查成为可能.
开发的技术增强了SMLM在生物研究方面的能力.

关键词:

CP: 图像处理图像处理这就是DNA-PAINT.SMLMLM SMLMLM 这是一个很大的问题.这是一场风暴.交叉语音交叉语音交叉语音交叉语音交叉语音 dSTORM 这是一个风暴.光是一种光.多色多彩的颜色.多目标多目标.频谱去混合的使用.超高分辨率的显微镜显微镜.

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