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相关概念视频

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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在同步多色单分子定位显微镜中评估交叉声谱.

Karoline Friedl1, Adrien Mau2, Fanny Boroni-Rueda3

  • 1Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France; Abbelight, 191 Avenue Aristide Briand, 94230 Cachan, France.

Cell reports methods
|September 26, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了更快的多色单分子局部化显微镜 (SMLM) 方法. 在dSTORM (SD-dSTORM) 中进行光谱解为先进的多色SMLM调查提供了强大的选择.

关键词:
CP: 图像处理 图像处理这就是DNA-PAINT.SMLMLM SMLMLM 这是一个很大的问题.这是一场风暴.交叉语音 交叉语音 交叉语音 交叉语音 交叉语音dSTORM 这是一个风暴.光是一种光.多色多彩的颜色.多目标多目标.频谱去混合的使用.超高分辨率的显微镜显微镜.

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科学领域:

  • 生物物理学的生物物理.
  • 光学显微镜的使用方法
  • 纳米技术 纳米技术

背景情况:

  • 单分子定位显微镜 (SMLM) 实现了纳米尺度的分辨率.
  • 像STORM和DNA-PAINT这样的技术是已建立的SMLM方法.
  • 更快的多色SMLM对于复杂的生物成像至关重要.

研究的目的:

  • 实施和评估两种新的方法,以实现更快的多色SMLM.
  • 评估dSTORM (SD-dSTORM) 中光谱脱杂的交叉声和生物相关性.
  • 将这些方法扩展到3D和三色成像.

主要方法:

  • 开发了同时进行的双色DNA-PAINT (S2C-DNA-PAINT) 和光谱除混合的dSTORM (SD-dSTORM).
  • 在两台摄像机之间分割发射的光,用于同时成像.
  • 通过使用DNA纳米制造器和标记细胞评估SD-dSTORM交叉声.
  • 评估了交叉语音对检测亚衍射模式的影响.

主要成果:

  • S2C-DNA-PAINT 作为低交叉声的参考.
  • 在各种样本类型中评估了SD-dSTORM交叉通话.
  • 评估了对生物模式的交叉影响.
  • 证明了成功扩展到3D采集和三色成像.

结论:

  • 光谱解混 (SD-dSTORM) 是一种可行的多功能方法,用于多色SMLM.
  • 这些进步使纳米尺度成像中的更强大的调查成为可能.
  • 开发的技术增强了SMLM在生物研究方面的能力.