基于小分子有机探针的10nm以下分辨率的三维多色光学纳米镜
Yubing Han1, Shijie Tu2, Wenwen Gong3
1College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Cell reports methods
|September 26, 2023
概括
研究人员使用小分子探针开发了一种新的多色扩张刺激的排放耗尽技术. 这种方法克服了扩展显微镜中的光子损失挑战,使得10nm以下的分辨率可用于可视化细胞结构和蛋白质相互作用.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜技术 显微镜技术
- 分子成像学分子成像学
背景情况:
- 扩展显微镜 (ExM) 由于光子损失,在实现纳米尺度分辨率方面面临限制.
- 细胞内蛋白质及其相互作用的高分辨率成像对于理解细胞功能至关重要.
研究的目的:
- 开发一种新的多色扩张刺激减排 (ExSD) 技术,以克服ExM的解决方案挑战.
- 为了实现高密度和高强度标签,以改善细胞超结构的可视化.
主要方法:
- 开发使用小分子探针的多色ExSD技术.
- 应用该技术可视化微纤维,溶解体和线粒体.
主要成果:
- 在细胞结构的成像中实现了10nm以下的分辨率.
- 证明了高标签密度和强度,克服了光子损失问题.
- 启用了细胞内蛋白质及其空间关系的详细可视化.
结论:
- 开发的ExSD技术显著提高了扩展显微镜中的分辨率.
- 这一进步为细胞生物学提供了新的见解,允许详细观察亚细胞成分和蛋白质相互作用.
相关概念视频
01:37Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
01:28Three-Dimensional Microscopy in Microbiology
60
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
60
01:18Imaging Biological Samples with Optical Microscopy
4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.8K