在海洋水域追踪SARS-CoV-2的足迹
Giuseppina La Rosa1, P Mancini1, M Iaconelli1
1National Center for Water Safety (CeNSia), Istituto Superiore di Sanità, Rome, Italy.
The Science of the total environment
|September 26, 2023
概括
严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 在远离海岸线的偏远海洋水域被检测到. 这项研究在16.3%的海洋样本中发现了SARS-CoV-2RNA,这表明它存在于开放海洋环境中.
科学领域:
- 环境病毒学 环境病毒学
- 海洋微生物学 海洋微生物学
- 公共卫生监督 公共卫生监督
背景情况:
- 废水监测一直是水生环境中检测SARS-CoV-2的主要方法.
- 在遥远的海洋水域中,SARS-CoV-2的存在和范围在很大程度上仍未被探索.
研究的目的:
- 为了调查SARS-CoV-2在远离海岸线的海洋和海洋水域的发生情况.
- 为了确定SARS-CoV-2RNA在这些偏远水生环境中的流行率和度.
主要方法:
- 收集了来自不同海洋地区的43个大容量 (500升) 海水样本.
- 使用的分子方法:实时RT-qPCR和嵌套PCR,其次是片测序.
主要成果:
- 在43个样本中有7个 (16.3%) 中检测到SARS-CoV-2RNA,特别是在大西洋和地中海.
- 检测到的度在每100升基因组拷贝6至470个之间.
- 在阳性样本中测序与Omicron变异一致的鉴定突变.
结论:
- 在偏远的海洋水域中存在SARS-CoV-2RNA,超出了沿海废水的影响范围.
- 研究结果强调需要对海洋生态系统中的病毒进行更广泛的环境监测.
- 需要进一步研究以确定SARS-CoV-2在海洋环境中的传染性.
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