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多种耐药性Acinetobacter baumannii的分子类型分类从ERIC-PCR和MLVA的临床标本中分离出来

Leili Osanloo¹, Habib Zeighami², Fakhri Haghi³

¹Department of Microbiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

Current microbiology

|September 26, 2023

概括

宝曼尼菌 (Acinetobacter baumannii) 导致严重的医院感染. 像ERIC-PCR和MLVA这样的分子排型方法揭示了这些关键病原体的高多药耐药性和遗传多样性.

科学领域:

医学微生物学医学微生物学
传染性疾病传染性疾病
遗传学是一个遗传学.

背景情况:

宝曼尼菌 (Acinetobacter baumannii) 是一种阴性细菌,是导致重大医院感染和高患者死亡率的细菌.
有效的分子类型识别对于理解克隆关系和管理A. baumannii.爆发至关重要.

研究的目的:

研究来自ICU患者的Acinetobacter baumannii分离物的抗生素耐药性模式和遗传多样性.
评估肠细菌重复性基因间共识聚合酶连锁反应 (ERIC-PCR) 和多个位置变量数量并列重复 (MLVA) 分析对A. baumannii基因型定型的有用性.

主要方法:

用100个A. baumannii分离物的磁盘扩散测试来评估抗生素耐药性.
用ERIC-PCR和MLVA分子类型化技术分析了遗传多样性.
确定了对氨基糖化物耐药性的基因.

主要成果:

所有分离物体都表现出多药性耐药性 (MDR),其中32%对所有测试的抗生素具有耐药性,91%具有广泛耐药性 (XDR).
观察到高水平的氨基糖化物耐药性,与特定的修饰酶相关 (aac(6') -Ib, ant(3′′) -I, aph(2′′) -Id).
埃里克-PCR发现了4个不同的集群,而MLVA发现了9个不同的集群,表明了显著的遗传多样性.

结论:

来自ICU患者的A. baumannii分离物显示出高水平的抗生素耐药性,包括氨基糖化物.
埃里克PCR和MLVA是评估A. baumannii的遗传多样性和克隆相关性的宝贵工具,有助于疫情管理.

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