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血清代谢物分析揭示了使用基于LC/MS的代谢资料的败血症患者的代谢特征:一个横截面研究

Jinliang Peng¹, Chongrong Qiu¹, Jun Zhang¹

¹Department of Emergency, The Affiliated Ganzhou Hospital of Nanchang university, Ganzhou, Jiangxi Province, 341000, China.

BMC medical genomics

|September 26, 2023

概括

早期发现败血症对于患者的治疗结果至关重要. 这项研究确定了特定的血清代谢物,如PS (22:0/22:1(11Z)) 和C17-Sphinganine,作为及时毒症诊断和干预的潜在生物标志物.

科学领域:

* 生物化学 * 生物化学
* 临床诊断临床诊断临床诊断
* 系统生物学系统生物学

背景情况:

* 败血症需要早期干预以改善患者的预后.
*确定可靠的败血症生物标志物对于及时诊断至关重要.
*血清代谢学为生物标志物发现提供了一个有前途的方法.

研究的目的:

* 通过代谢概况识别,识别出潜在的血清败血症生物标志物.
*为了区分败血症患者和健康个体之间的代谢特征.
* 探索已识别的代谢物与败血症发病的关联.

主要方法:

*分析了来自败血症患者和健康对照者的血清样本.
* 液体染色学-质谱学 (LC-MS) 用于代谢分析.
* 生物信息技术被用于分析代谢数据并识别差异表达的代谢物.

主要成果:

*在败血症患者和对照人群之间观察到血清代谢物度的显著差异.
*在败血症患者中观察到酸盐胆 (PCs),酸盐 (PS),酸盐乙醇胺 (LysoPEs) 和酸盐胆 (LysoPCs) 的水平降低.
*在败血症患者中发现了肌素,C17-斯芬甘素和PS{22:0/22:1}{11Z}的升高水平,诊断准确度高 (AUC>0.9).

结论:

* 特定的代谢物,包括PS (22:0/22:1(11Z)) 和C17-Sphinganine,显示出作为败血症诊断标记物的潜力.
*代谢分析有助于理解败血症的发病因子,包括mTOR和NF-κB信号传递等途径.
*这些生物标志物的早期识别可以促进及时的败血症诊断和干预,改善患者的治疗结果.

关键词:

生物标志物生物标志物液体染色学-质谱学-质谱学这是一个代谢学 (metabonomic) 体系.酸胆酸盐是一种酸胆.败血症这是一种败血症.

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