circELMOD3通过海绵化miR-6864-5p和直接结合来抑制HCC进展而增加和稳定TRIM13
Mingshuang Lai1,2, Meiliang Liu1,2, Deyuan Li1,2
1School of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China.
iScience
|September 27, 2023
概括
循环RNA ELMOD3 (circELMOD3) 在肝细胞癌 (HCC) 中下调,并作为一个有前途的诊断生物标志物. circELMOD3通过调节miR-6864-5p/TRIM13/p53通路来抑制瘤生长并促进细胞亡.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 循环RNAs (circRNAs) 越来越多地与肝细胞癌 (HCC) 的病原发生有关.
- 了解 circELMOD3 等特定 circRNA 的作用,对于 HCC 的诊断和治疗至关重要.
研究的目的:
- 研究肝细胞癌中circelMOD3的诊断价值,功能作用和潜在机制.
- 探索circELMOD3作为一种潜在的HCC检测的非侵入性生物标志物.
主要方法:
- RNA测序以识别HCC组织中差异表达的circRNAs.
- 定量实时PCR和血测定用于circELMOD3检测.
- 在体外和体外功能测试以评估增殖和亡.
- 为了阐明分子机制,RNA反感性净化 (RAP) 和 luciferase 记者测试.
主要成果:
- circELMOD3在HCC组织中显著下调,与临床病理特征相关.
- 血circELMOD3显示出高灵敏度和特异性,作为区分HCC与健康对照的生物标志物.
- circELMOD3的过度表达抑制了HCC细胞的增殖,并在体外和体内引起了细胞灭亡.
- circELMOD3 作为 miR-6864-5p 的分子海绵,上调 TRIM13 的表达并稳定其 mRNA.
- 通过TRIM13,circELMOD3同样激活了p53信号通路.
结论:
- circELMOD3 是一个潜在的非侵入性诊断生物标志物,用于HCC.
- circELMOD3通过抑制增殖和诱导亡,在HCC中起到瘤抑制作用.
- 环ELMOD3/miR-6864-5p/TRIM13/p53轴代表了HCC的新型治疗标.
更多相关视频
相关概念视频
02:33Hedgehog Signaling Pathway
7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
02:34Inhibition of Cdk Activity
4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
01:23Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K