Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Preparation and Hypoglycemic Activity of Enzymatically Hydrolyzed Peptides From the Swim Bladder of <i>Pangasius bocourti</i>.

Food science & nutrition·2026
Same author

Stage-of-Action Characterization of a Non-Sulfated Heteropolysaccharide from <i>Gracilaria lemaneiformis</i> Against Dengue Virus Serotype 2.

Viruses·2026
Same author

Polymer-regulated crystallization enables scalable, high-performance heterostructured perovskite luminescent optoelectronic fibers.

Science bulletin·2026
Same author

Fluorescence detection strategy based on DNA tetrahedron and ATP-assisted signal amplification for intracellular hypochlorous acid imaging.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Confinement-Regulated Crystal Phase Engineering Enables Structured Semiconductor Fiber Systems for Plant Transpiration Dynamics Monitoring.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

<i>Laminaria japonica</i> polysaccharide mitigates acute neuroinflammation in cerebral ischemia-reperfusion injury through Csf3-modulated pathways.

Frontiers in immunology·2026

相关实验视频

Updated: Jul 15, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.2K

基于生物感应的双光子功能核酸探针.

Kefeng Wu1,2, Changbei Ma3, Yisen Wang1,2

  • 1GBA Branch of Aerospace Information Research Institute, Chinese Academy of Sciences, Guangzhou 510700, China.

Biosensors
|September 27, 2023
PubMed
概括

功能核酸 (FNA) 探针为检测提供了优势. 双光子 (TP) 激发增强了FNA探头,改善了生物医学传感,减少了光损伤,与单光子方法相比,更好地透了组织.

关键词:
DNA纳米技术 DNA纳米技术这是一个RNARNARNARNARNA.生物成像是一种生物成像.生物分子生物分子.光探测器的光探测器通过光激活的光激活.两个光子的两个光子.

更多相关视频

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.0K

相关实验视频

Last Updated: Jul 15, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.2K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.0K

科学领域:

  • 生物医学传感和成像技术
  • 分子探测器和诊断仪器

背景情况:

  • 功能核酸 (FNA) 探针是环境,食品,临床和成像应用中的多功能工具.
  • 传统的单光子 (OP) FNA探头面临着诸如光损伤和生物组织中光线透率低下等局限性.
  • 基于近红外 (NIR) 光的双光子 (TP) 激发为FNA探测器提供了相对于OP激发的优势.

研究的目的:

  • 审查两光子 (TP) 激发和激活的功能核酸 (FNA) 探针的最新进展.
  • 详细介绍基于TP的FNA探针在生物分子检测中的应用.
  • 讨论基于TP的高性能FNA探头的亮点,局限性和设计策略.

主要方法:

  • 关于TP激发和激活FNA探针的文献综述.
  • 分析TP探针的特性:较低的组织吸收/自发光,减少光损伤/光变白,以及更高的空间分辨率.
  • 在生物医学传感中,基于TP的FNA探头与基于OP的传统FNA探头的比较.

主要成果:

  • 基于TP的FNA探头在生物医学传感方面表现出卓越的性能,这是由于其增强的光学特性.
  • TP激发使组织透更深,并降低光毒性,这对于体内应用至关重要.
  • 基于TP的FNA探测器为生物分子检测提供了更好的空间分辨率和信号噪声比.

结论:

  • 以TP激发和激活的FNA探头比基于OP的生物医学传感探头具有显著的进步.
  • TP激发的独特特性使生物分子检测更有效,更少侵入性.
  • 基于TP的FNA探针的进一步开发对于扩大其生物和临床应用至关重要.