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一个新型的双细菌印制聚合物传感器,用于高度选择性和快速检测致病细菌

Xiaoli Xu¹, Xiaohui Lin¹, Lingling Wang¹

¹College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

|September 27, 2023

概括

一个新的双细菌印制的聚合物传感器快速和选择性地检测出病原细菌,如大肠杆菌O157:H7和金黄色. 这种具有成本效益的,可重复使用的传感器为提高食品安全提供快速制造和分析.

科学领域:

* 分析化学分析化学
* 材料科学材料科学
* 食品科学食品科学

背景情况:

* 检测致病细菌对于食品安全和公共卫生至关重要.
*现有的方法往往缺乏速度,灵敏度或选择性.
*需要新型传感器来快速,可靠地识别病原体.

研究的目的:

* 开发一种新的,可重复使用的,具有成本效益的阻抗计传感器,用于检测大肠杆菌O157:H7和金黄色细菌.
* 为了实现快速制造,高选择性和细菌检测的灵敏度.
* 为了证明传感器在真实食品样本中的实际应用.

主要方法:

*通过电聚合制造制造双细菌印制聚合物 (DBIP) 传感器.
* 模板移除和现场再生,以便重复使用.
* 阻抗测量测量用于选择性和敏感地检测目标细菌.

主要成果:

*DBIP传感器在20分钟内制造,没有空洞的印记部位.
* 快速的目标细菌重新结合 (15分钟) 和接口再生 (10分钟).
*高灵敏度 (检测极限~9 CFU mL-1) 和对相关菌株的选择性.
* 在果汁样本中成功应用,具有良好的回收率.

结论:

*DBIP传感器为快速,选择性和敏感的病原细菌监测提供了一个有前途的解决方案.
* 它的可重复使用性和成本效益提高了实际适用性.
* 传感器为确保食品安全和公共卫生保护做出了重大贡献.

关键词:

大肠杆菌埃舍里奇亚菌O157:H7 黄金葡萄球菌黄金葡萄球菌细菌检测检测细菌的检测双重细菌印记的聚合物电化学传感器是一种电化学传感器.有分子印记的聚合物.这是一种o-phenylenediamine.

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