碳氧甲基基托桑微凝用于持续输送万科米辛和长期抗菌效果
Mehtap Sahiner1,2, Aynur S Yilmaz2,3, Ramesh S Ayyala4
1Department of Bioengineering, Faculty of Engineering, Canakkale, Onsekiz Mart University Terzioglu Campus, Canakkale 17100, Turkey.
Gels (Basel, Switzerland)
|September 27, 2023
概括
碳氧甲基基托 (CMCh) 微凝被合成用于药物输送. 这些生物相容的微凝有效地加载和释放了万科米,显示出对大肠杆菌和黄金色杆菌的显著抗菌活性.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 纳米技术纳米技术
背景情况:
- 碳甲基 (CMCh) 是一种多功能多糖,以其生物相容性,生物降解性和水溶性而闻名.
- 其独特的功能组允许调节电荷特性,使其适合各种应用.
- CMCh的化能力和功能组有利于开发先进材料.
研究的目的:
- 为了合成和表征碳氧甲基基托 (CMCh) 微凝.
- 为了评估载药能力和Vancomycin载荷的CMCh微凝 (Van@CMCh) 的抗菌疗效.
- 探索CMC微凝作为药物输送系统的潜力.
主要方法:
- 在微乳液环境中,CMCh微凝被合成使用乙硫 (DVS) 作为交联剂.
- 使用SEM分析了微凝大小,泽塔潜力和形态学.
- 使用L929纤维细胞评估生物相容性.
- 药物加载和释放研究使用万科米辛 (Van) 进行.
- 使用MIC和磁盘扩散试验对大肠杆菌和黄金色杆菌进行了抗菌活性评估.
主要成果:
- 成功合成了大小从一到几十微米的球形CMCh微凝.
- 微凝的同电点被确定为pH 4.4.4.
- CMCh微凝表现出极好的生物相容性,细胞活力为96.5±1.5%在1mg/ml的剂量下.
- 在24小时内,Van@CMCh微凝显示出显著的抗菌活性,MIC值为68.6微克/毫升针对大肠杆菌和7.95微克/毫升针对黄金色杆菌.
- 观察到长期抑制细菌生长,长达72小时,特别是针对黄金色细菌.
结论:
- CMCh微凝可以有效地合成,具有受控的特性.
- 开发的微凝具有生物相容性,适用于药物输送应用.
- 范@CMCh微凝显示出作为具有持续释放性质的抗菌剂的有希望的潜力.
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