病毒和细胞端粒酶RNA具有宿主特异性的抗亡功能
Ahmed Kheimar1,2, Laetitia Trapp-Fragnet3, Andelé M Conradie1
1Institute of Virology, Freie Universität Berlin , Berlin, Germany.
Microbiology spectrum
|September 27, 2023
概括
人类端粒酶RNA (hTR) 在马雷克病病毒模型中不驱动瘤形成,尽管其结构保留了. 病毒和细胞端粒酶RNA都表现出对宿主特异的抗亡功能,这对瘤发生至关重要.
科学领域:
- 病毒学 病毒学
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 人类端粒酶RNA (hTR) 在癌症中过度表达,并独立于端粒酶抑制细胞亡.
- 马雷克病病毒 (MDV) 是一种高度瘤性疹病毒,它编码一种病毒端粒酶RNA (vTR),对MDV诱导的瘤产生至关重要.
- vTR与hTR共享保留的功能域,表明潜在的功能重叠.
研究的目的:
- 为了调查hTR是否可以在疹病毒诱导的瘤发生的自然模型中驱动瘤形成.
- 确定vTR,hTR和端粒酶RNA (cTR) 的抗亡活性.
- 为了阐明端粒酶RNAs在病毒瘤发生中的宿主特异性功能.
主要方法:
- 产生一种复合MDV,其中vTR被hTR取代.
- 评估复合病毒的hTR表达和复制.
- 在人类和细胞中评估vTR,hTR和cTR的抗亡功能.
主要成果:
- hTR在复合MDV中得到了有效的表达,并且没有影响病毒复制.
- 尽管结构性保护,但hTR未能补充vTR的损失,促进MDV诱导的瘤发生.
- hTR在人类细胞中表现出有效的抗亡作用,但在细胞中没有,而vTR和cTR显示出宿主特异性的抗亡功能.
结论:
- 在MDV模型中,hTR没有促进瘤的特性.
- vTR,cTR和hTR显示保存但宿主特定的抗亡功能.
- 端粒酶RNAs的这些宿主特异性抗亡作用对病毒瘤发生至关重要,并为癌症治疗提供翻译性见解.
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