素是尼的短自然变体,表现出强大的抗菌活性对主要病原体,尽管缺少两个C端的宏循环
Longcheng Guo1, Joseph Wambui2, Chenhui Wang1
1Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen , Groningen, the Netherlands.
Microbiology spectrum
|September 27, 2023
概括
素是一种新型的抗生素变体,通过抑制细菌细胞壁合成,表现出与素相似的强有力的抗菌活性. 生物工程增强了其稳定性,使其成为食品和健康应用的有希望的候选者.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 抗菌研究 抗菌研究
背景情况:
- 尼辛是一种广泛使用的食品级抗菌素,具有抗菌性质,通过细胞壁合成抑制和毛孔形成起作用.
- 素是来自*Clostridium estertheticum*的天然短变体,缺乏形成毛孔的域,但其抗菌潜力以前没有被描述.
- 抗生素耐药性病原体的增加需要发现新型抗菌剂.
研究的目的:
- 为了异质地表达和表征素的抗菌活性和物理化学性质.
- 阐明素的作用方式,重点关注其结合相互作用和孔隙形成能力.
- 评估素的稳定性,并探索生物工程策略,以提高其适用性.
主要方法:
- 在 *Lactococcus lactis* 中素的异质表达.
- 抗微生物活性测定针对广泛的细菌.
- 脂质II和利波泰可酸结合试验.
- 光显微镜检测毛孔形成.
- 在各种pH和温度条件下进行稳定性测试,以及对素的敏感性.
- 一种抗素的类似物,Cesin R15G.的生物工程.
主要成果:
- 异质表达的氨酸表现出强大和广泛的抗菌活性,与尼氨酸相比.
- 素的活性与与铁酸的脂质II结合和静电相互作用有关,抑制细胞壁合成.
- 在其自然形式中,素缺乏孔隙形成能力,但在不同的pH值和温度下表现出高稳定性.
- 一种生物工程变异,Cesin R15G,显示出对素降解的耐药性,同时保持完整的抗菌作用力.
结论:
- 素是一种新型,强效的lantibiotic,通过细胞壁合成抑制而有效抑制细菌生长,而无需孔隙形成.
- 氨酸的结构特性和稳定性使其成为开发新抗菌剂的有希望的支架.
- 生物工程素变体,如素R15G,可以克服诸如酶降解等局限性,增强其食品和健康应用的潜力.
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