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在actinobacterium Corynebacterium glutamicum 中,混合型pyruvate/2-oxoglutarate脱酶复合物的细胞局部化

Lea Sundermeyer¹, Jan-Gerrit Folkerts¹, Benita Lückel¹

¹IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum Jülich , Jülich, Germany.

Microbiology spectrum

|September 27, 2023

概括

这项研究表明,actinobacteria中的混合型pyruvate/2-oxoglutarate脱酶 (PDH-ODH) 复合体局部化到细胞极和细胞中部. 这种空间组织表明有结构的新陈代谢,不同于典型的细胞质酶分布.

科学领域:

细菌生理学细菌生理学
分子细胞生物学分子细胞生物学
生物化学生物化学

背景情况:

细菌中央代谢中的酶通常被认为在细胞质中均分布.
代谢酶的特定局部化是不太了解的,特别是在actinobacteria.
混合型的pyruvate脱酶/2-oxoglutarate脱酶 (PDH-ODH) 复合体是actinobacteria独有的.

研究的目的:

为了研究动态细菌中混合PDH-ODH酶复合物的细胞局部化.
要确定这个复合体是否在细菌细胞内表现出空间组织.
探索这种局部化对细菌新陈代谢的影响.

主要方法:

在Corynebacterium glutamicum中使用光显微镜.
采用光蛋白融合 (mVenus,mCherry) 来标记PDH-ODH子单元 (AceE,AceF,Lpd,OdhA). 使用光蛋白融合 (mVenus,mCherry) 来标记PDH-ODH子单元 (AceE,AceF,Lpd,OdhA). 使用光蛋白融合 (mVenus,mCherry) 来标记PDH-ODH子单元 (AceE,AceF,Lpd,OdhA).
观察到子单元和调控蛋白 (OdhI) 的局部化模式.

主要成果:

混合PDH-ODH复合物的所有四个子单元在Corynebacterium glutamicum的细胞极和细胞中部的不同点上共同定位.
抑制蛋白OdhI在非酸化时与极点的复合物局部化,但在酸化时是扩散的.
代谢相关的酶 (异酸脱酶,谷氨酸脱酶) 显示出一个均的细胞质分布.

结论:

杂交的PDH-ODH复合体存在,并且在actinobacteria中表现出特定的极性和中细胞局部化.
这种空间组织表明一个分隔的新陈代谢,可能受到核状排斥的影响.
这些发现挑战了在细菌中央代谢中同质酶分布的假设,并开辟了新的研究途径.

关键词:

2 - 氧格卢酸脱酶复合物在Corynebacterium glutamicum的研究中,我们发现这是一种ODH抑制蛋白OdhI.细胞局部化细胞局部化光显微镜的光显微镜.酸盐脱基酶复合物

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